ABBOTTNUTRITIONDIVISION
TITLE
PAGENO.
Determination of TransVitaminK
1
byHPLC andFluorescenceDetection
2 of 14
A.
SAFETY
ALLREAGENTSANDEQUIPMENTSHOULDBEHANDLED INAMANNERCONSISTENTWITH
SAFELABORATORYPRACTICE. REAGENTSANDSAMPLESSHOULDBETREATEDWITHTHE
RESPECTANDCARETHATANYCHEMICALREQUIRES. REAGENTSWHICHAREALLOWED
TOCOME INCONTACTWITHTHESKIN, EYES, ORCLOTHINGSHOULDBEFLUSHED
IMMEDIATELYWITHLARGEVOLUMESOFWATER. IMMEDIATEMEDICALATTENTION
SHOULDBEOBTAINED IFANYREAGENT IS INGESTEDORALLOWEDTOCOME IN
CONTACTWITHTHEEYES.
ALLOFTHESOLVENTSUSED INTHISMETHODAREHIGHLYFLAMMABLEANDSHOULD
BEKEPTAWAYFROMFLAMESANDOTHER IGNITIONSOURCES
METHANOL ISATOXICCUMULATIVEPOISONTHATCANCAUSEBLINDNESS.
ACETICACID ISASTRONGOXIDIZER. DIRECTCONTACTMAYCAUSE IRRITATIONTOSKIN
ANDEYES.
ZINCPOWDER ISSELF-HEATINGANDMAYCATCHFIRE. ZINCPOWDERSHOULDNOTBE
ALLOWEDTOCOME INTOCONTACTWITHWATER. CONTACTWITHWATERMAY
RELEASEFLAMMABLEGASESWHICHMAYSPONTANEOUSLY IGNITE.
B.
THEORY
1. VitaminK
1
(also known as phylloquinone and phytonadione) occurs naturally in the trans form as
yellow viscous oil. During synthesis of vitaminK
1
both the cis and trans isomers are formedwith the
trans isomer being themajor product. VitaminK
1
is insoluble inwater and sparingly soluble in
methanol and ethanol. It is soluble in vegetable oils andorganic solvents such as pentane, hexane,
isooctane, and 2-propanol. Themeltingpoint of vitaminK
1
is -20
C and itsmolecularweight is
450.71. VitaminK
1
has 5 ultraviolet absorptionmaximumwhich are at 242, 248, 260, 269, and 325
nm. VitaminK
1
is stable to air, heat, oxidizing agents, andmoisture, but its activity is destroyed by
light (especiallyultraviolet radiation), reducing agents, and alkalies.
2. Good sources of vitaminK
1
are alfalfa, cabbage, cauliflower, green vegetables, tomatoes, cheese, dairy
products,meat, egg yolks, and canola and soyoil. VitaminK
1
is also found in bacteria and is
synthesized in the intestinal tract bymicroorganisms. Trans vitaminK
1
is biologically activewhile the
cis form has little, if any, activity.
O
O
CH
3
CH
2
CH CCH
2
CH
2
CH
2
CHCH
2
CH
2
CH
2
CHCH
2
CH
2
CH
2
CHCH
3
CH
3
CH
3
CH
3
CH
3
VitK-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
