Silliker Laboratories of IL, Crete, IL60417
AMethodof VitaminKAnalysis (cis and trans) in infant formulaand adult
Nutritional Formulas
Principle:
An aliquot of the sample ismixedwith internal standard. Fat in the sample hydrolyzed
by lipase. VitaminK in the sample after lipase treatment is extracted in solvent. The
reconstitutedextyract is analyzed by LC-MS/MS for vitaminK.
AnalyteScope:
VitaminK1 cis and trans
VitraminK2-4 cis and trans
ApplicableMatrices:
Infant formula and adult Nutritional Formulas
Chemicals:
a.) Lipase
–
Sigma #L1754-100G
b.) Potassiumphosphatemonobasic
c.) 10NNaOH: 10NNaOH obtained fromFischer (Fisher #746616)
d.) K1 IS
–
Aldrich part#705470; 2-methyl-3-phytyl-1,4-naphthoquinone;
VitaminK1-d7 (5,6,7,8-d4, 2-methyl-d4)
e.) VitaminK1Standard
–
Chromadex #ASB-00022816-001
f.) VitaminK2-4Standard
–
Chromadex #ASB-00022821-025
g.) Iso-Octane
–
Fisher #O2964
h.) Ethanol
–
Sigma#459836-2L
i.) Iso-propanol (IPA)
j.) Methanol (MeOH)
Supplies:
a.) ShimadzuUFLC system coupledwithAPI 3200TripleQuadMass Spectrometer
b.) Column: YMCCAROTENOIDS-32.0X150mmPart#CT99S03-1502WT
a.) 50ml Centrifuge tubes
b.) 16x125 glass culture tubes
c.)
Turbo evaporator
d.)
Centrifuge
e.)
Shaker
f.)
Water bath (37
o
C)
Reagents:
VitK-01
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
