Method Submissions-5th Set of Nutrients_6-30-14 - page 199

Silliker Laboratories of IL, Crete, IL60417
LCConditions
Column: YMCCAROTENOIDS-32.0X150mmPart#CT99S03-1502WT
Fisher#50-900-05344.
MobilePhase: A: 5mMAmmoniumAcetate inWater
B: 5mMAmmoniumAcetate in75:25MeOH:IPA
Gradient: Time
%A
%B
FlowRate (mL/min)
0.10
10
90
0.4
5.00
0
100
0.4
10.00
0
100
0.4
10.10
10
90
0.4
15.00
SystemController
STOP
Temperature: 35
o
C (column compartmentwill not cool below35
o
C)
InjectionVolume: 10uL
StopTime: 15min
MSConditions
IonSource: positive
Cur: 20.00
IS: 5500
TEMP: 350
GS1: 35.00
GS230.00
ihe: on
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