ABBOTTNUTRITIONDIVISION
TITLE
PAGENO.
Determination of TransVitaminK
1
byHPLC andFluorescenceDetection
4 of 14
p) Helium sparge tubing and filtering assembly
q)
MagneticStirrer;VariomagTelesystemHP 60S part#HP 46040Uwith tube rack part#HP 86029
and egg-shapedTeflon stirringbars part#PI20110 or equivalent
r)
Pipettes, disposable glass Pasteur pipettes
s)
Pipettes, volumetric (ClassA): assorted sizes
t)
Pipetter:mechanical variable volume pipetter (0.5-5.0mLs)
u)
Repeatingpipette : 5mL and 25mLor equivalent
v)
Vortexmixer
w)
Weighingdish, glass
x)
Weigh boats, small, plastic
y)
Yellow lights or yellow shieldswith a cutoff of 440 nm
3. Chemicals/Standards
Unless otherwise noted, the following chemicals should be stored at 2-30
C in a tight inert container is
appropriate unless otherwise stated. Their expiration date is one year after the date they are first
opened. Upon expiration the chemicalsmust be either discardedor re-evaluated. Regardless of
expiration or re-evaluation dates, discontinue use of any chemicals or solutionswhenever indications
of contamination, chemical degradation, or changes in concentration are evident.
a)
AceticAcid, ACS
b)
Control Sample, Current Lot
c)
Helium, ZeroGrade or equivalent
d)
Isooctane (2,2,4-trimethylpentane), HPLCGrade
e)
Isopropanol (isopropyl alcohol), HPLCGrade
f)
Methanol, HPLCGrade
g)
Phytonadione (VitaminK
1
), USPReference, Official Lot. Store under yellow lightingor in the
darkper label instructions
h)
LaboratoryWater
i)
ReagentAlcohol, ACS
j)
SodiumAcetateAnhydrous, ACS
k)
Zinc, Sigma-AldrichP/N 324930, <150micron, 99.995%or equivalent
l)
ZincChloride, ACS
D.
STANDARDANDSOLUTIONPREPARATION
VitK-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
