SimultaneousDetermination of SevenWater SolubleVitamins inProducts byLC-MS/MS
Page
18
of
20
ii)
Mass SpectrometryConditions - SeeTablesA4, A5 and, A6
TableA4. Mass analysis parameters
Compound
Function
No.
Start
(min)
End
(min)
SRMTransition
Typ. RT
(min)
Mass SpecConditions
Molecula
r Ion
Fragment
Ion
Cone
Voltage
CE
Voltage
Dwell Time
(sec)
Niacinamide
1
0.2 2.4
122.9
79.9
1.5
30
15
0.040
Niacinamide (C)
1
0.2 2.4
122.9
96.1
1.5
30
15
0.040
2
H
4
-Niacinamide
1
0.2 2.4
127.0
100.1
1.5
30
15
0.040
2
H
4
-Niacinamide (C)
1
0.2 2.4
127.0
84.1
1.5
30
15
0.040
Pyridoxine
2
2.4 3.5
170.0
151.9
3.0
30
12
0.025
Pyridoxine (C)
2
2.4 3.5
170.0
134.0
3.0
30
20
0.025
13
C
4
-Pyridoxine
2
2.4 3.5
174.0
155.9
3.0
30
12
0.025
13
C
4
-Pyridoxine (C)
2
2.4 3.5
174.0
138.0
3.0
30
20
0.025
PantothenicAcid
2
2.4 3.5
220.1
90.0
2.7
12
12
0.025
PantothenicAcid (C)
2
2.4 3.5
220.1
124.0
2.7
12
20
0.025
13
C
3
,
15
N-Pantothenic
Acid
2
2.4 3.5
224.0
94.0
2.7
12
12
0.025
13
C
3
,
15
N-Pantothenic
Acid (C)
2
2.4 3.5
224.0
126.0
2.7
12
20
0.025
Biotin
3
3.5 4.6
245.1
123.0
4.2
22
25
0.020
Biotin (C)
3
3.5 4.6
245.1
97.0
4.2
22
25
0.020
13
C
5
-Biotin
3
3.5 4.6
250.1
126.0
4.2
22
25
0.020
13
C
5
-Biotin (C)
3
3.5 4.6
250.1
97.0
4.2
22
25
0.020
Thiamine
3
3.5 4.6
265.1
121.9
3.8
24
12
0.025
Thiamine (C)
3
3.5 4.6
265.1
81.0
3.8
24
30
0.025
13
C
4
-Thiamine
3
3.5 4.6
269.0
122.0
3.8
24
12
0.025
13
C
4
-Thiamine (C)
3
3.5 4.6
269.0
81.0
3.8
24
30
0.025
FolicAcid
3
3.5 4.6
442.1
295.0
4.1
20
15
0.020
FolicAcid (C)
3
3.5 4.6
442.1
176.0
4.1
20
35
0.020
FalseTransition*
3
3.5 4.6
445.3
295.0
4.1
20
20
0.020
13
C
5
-FolicAcid
3
3.5 4.6
447.1
295.0
4.1
20
15
0.020
13
C
5
-FolicAcid (C)
3
3.5 4.6
447.1
176.1
4.1
20
35
0.020
Riboflavin
4
4.6 5.5
376.9
242.9
4.8
40
20
0.080
Riboflavin (C)
4
4.6 5.5
376.9
172.2
4.8
40
35
0.080
13
C
4
,
15
N
2
-Riboflavin
4
4.6 5.5
383.0
248.9
4.8
40
20
0.080
13
C
4
,
15
N
2
-Riboflavin (C)
4
4.6 5.5
383.0
175.0
4.8
40
35
0.080
(C) Confirmatory ion
*False transition is added toprevent instrumental cross-talkbetween the native and ISof folic acid
BIO-03
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
