SimultaneousDetermination of SevenWater SolubleVitamins inProducts byLC-MS/MS
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Instrument OperatingConditions
i)
UPLCConditions
TableA3. UPLC instrument configuration details
Instrument Components
BinarySolventManager / SampleManager / 4 position
ColumnManager
MobilePhaseA1
0.05%FA, 0.04%HFBA inwater
MobilePhaseB1
5mM ammoniumhydroxide inmethanol
FlowRate
150µL/min (no split)
Column
WatersAcquityUPLCHSS-T3 1.8µm1.0 x 100mmPart
#186003536
ColumnTemperature
60
C
InjectionType
10µL full loop injection
LoopVolumeOverfill
Automatic
SampleTemperature
Ambient
InjectorWashSettings
StrongWash - Solvent
Methanol
StrongWash –Volume
1000µL
WeakWash –Solvent
Water
WeakWash -Volume
2000µL
Gradient Program
Time
%A1
%B1
Curve
0.00
99.0
1.0
Initial
0.40
99.0
1.0
6
6.00
55.0
45.0
6
6.50
1.0
99.0
2
7.50
1.0
99.0
6
7.60
99.0
1.0
6
9.00
99.0
1.0
6
BIO-03
FORWORKINGGROUP/ERPUSEONLY
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