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B
ird
et al
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J
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AOAC I
nternational
V
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100, N
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1, 2017
89
Table 2016.07D. Comparative results for the detection of
Listeria
in 25 g raw chicken breast fillet test portions by the 3M
MDA 2–
Listeria
method versus USDA/FSIS MLG 8.09 in the collaborative study
Uninoculated control
Statistic
Matrix/
inoculation
level
Lab
CP
a
CC
b
C
c
R
d
C vs R
N
e
x
f
POD
CP
N x
POD
CC
N x
POD
C
N x
POD
R
dLPOD
C vs R
dLPOD
CP vs CC
Raw
chicken
breast fillet
1
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
2
12 1 0.08 12 1 0.08 12 1 0.08 12 0 0.00
0.08
0.00
3
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
4
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
5
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
6
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
7
12 1 0.08 12 0 0
.00 12 0 0.00 12 0 0.000.00
0.08
8
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
9
12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
10
g
12 0 0
.00 12 2 0.17 12 0 0.0012 0 0.00
0.00
–0.17
11
h
NA NA NA NA NA NA NA NA NA NA NA NA
NA
NA
12 12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
13 12 0 0
.00 12 0 0.00 12 0 0.0012 0 0.00
0.00
0.00
Labs
N x
POD
CP
N x
POD
CC
N x
POD
C
N x
POD
R
dLPOD
C vs R
dLPOD
CP vs CC
Estimate
All
132 2 0.02 132 1 0.01 132 1 0.01 132 0 0.00
0.01
0.01
LCL
i
0.00
0.00
0.00
0.00
–0.02
–0.03
UCL
j
0.06
0.04
0.04
0.03
0.04
0.05
s
r
0.12
0.09
0.09
0.00
LCL
0.11
0.08
0.08
0.00
UCL
0.16
0.16
0.16
0.16
s
L
k
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.05
0.04
0.04
0.16
s
R
0.12
0.09
0.09
0.00
UCL
0.11
0.08
0.08
0.00
LCL
0.14
0.10
0.10
0.23
P
T
l
0.5190
0.4338
0.4338
1.0000
Low inoculum level
Raw
chicken
breast fillet
1
12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58
0.00
0.00
2
12 9 0.75 12 9 0.75 12 9 0.75 12 7 0.58
0.17
0.00
3
12 10 0.83 12 10 0.83 12 10 0.83 12 5 0.42
0.41
0.00
4
12 9 0.75 12 9 0.75 12 9 0.75 12 4 0.33
0.42
0.00
5
12 8 0.66 12 8 0.66 12 8 0.66 12 5 0.42
0.22
0.00
6
12 10 0.83 12 9 0.75 12 9 0.75 12 6 0.50
0.15
0.08
7
12 8 0.66 12 6 0.50 12 6 0.50 12 6 0.50
0.00
0.16
8
12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58
0.00
0.00
9
12 8 0.66 12 9 0.75 12 8 0.66 12 7 0.58
0.08
–0.08
10
g
12 6 0.50 12 8 0.66 12 6 0.50 12 5 0.42
0.08
–0.16
MPN/test
portion
11
h
NA NA NA NA NA NA NA NA NA NA NA NA
NA
NA
12 12 7 0.58 12 7 0.58 12 7 0.58 12 4 0.42
0.16
0.00
13 12 5 0.42 12 5 0.42 12 5 0.42 12 6 0.50
–0.08
0.00
Labs
N x
POD
CP
N x
POD
CC
N x
POD
C
N x
POD
R
dLPOD
C vs R
dLPOD
CP vs CC
Estimate 0.66
All
132 88 0.67 132 86 0.65 132 85 0.64 132 64 0.48
0.16
0.02
LCL
0.51
0.58
0.57
0.56
0.40
0.04
–0.10
UCL 0.83
0.75
0.73
0.73
0.57
0.28
0.13
s
r
0.48
0.48
0.48
0.51
LCL
0.42
0.43
0.43
0.46
UCL
0.52
0.52
0.52
0.52