45 / 199
B
ird
et al
.:
J
ournal of
AOAC I
nternational
V
ol
.
100, N
o
.
2, 2017
461
Table 2016.08D. Comparative results for the detection of
L. monocytogenes
at three inoculum levels in 25 g raw chicken
breast fillet test portions by the 3M MDA 2 –
L. monocytogenes
method vs the USDA/FSIS MLG reference method in a
collaborative study
Statistic Matrix Laboratory
Candidate
presumptive
Candidate
confirmed
Candidate
result
Reference
method
Candidate vs
reference
n
a
x
b
POD
CP
n x
POD
CC
n x
POD
C
n x
POD
R
dLPOD
C
dLPOD
CP
Uninoculated control
Raw
chicken
breast fillet
1
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
2
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
3
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
4
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
5
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
6
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
7
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.08
8
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
9
12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
10
c
12 0 0.00 12 2
0.17
12 0
0.00 12 0 0.00
0.00
−0.17
11 NA
d
NA NA NA NA
NA NA NA NA NA NA NA
NA
NA
12 12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
13 12 0 0.00 12 0
0.00
12 0
0.00 12 0 0.00
0.00
0.00
Estimate
All
132 0 0.00 132 0
0.00 132 0
0.00 132 0 0.00
0.00
0.00
LCL
e
0.00
0.00
0.00
0.00
−0.03
−0.03
UCL
f
0.03
0.03
0.03
0.03
0.03
0.03
s
r
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.16
0.16
0.16
s
L
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.16
0.16
0.16
s
R
0.00
0.00
0.00
0.00
UCL
0.00
0.00
0.00
0.00
LCL
0.23
0.23
0.23
0.23
P
T
1.0000
1.0000
1.0000
1.0000
Low inoculum level
Raw
chicken
breast fillet
1
12 7 0.58 12 7
0.58
12 7
0.58 12 7 0.58
0.00
0.00
2
12 9 0.75 12 9
0.75
12 9
0.75 12 7 0.58
0.17
0.00
3
12 10 0.83 12 10
0.83
12 10 0.83 12 5 0.42
0.41
0.00
4
12 9 0.75 12 9
0.75
12 9
0.75 12 4 0.33
0.42
0.00
5
12 8 0.66 12 8
0.66
12 8
0.66 12 5 0.42
0.22
0.00
6
12 9 0.75 12 9
0.75
12 9
0.75 12 6 0.50
0.15
0.08
7
12 7 0.58 12 6
0.50
12 6
0.50 12 6 0.50
0.00
0.16
8
12 7 0.58 12 7
0.58
12 7
0.58 12 7 0.58
0.00
0.00
9
12 8 0.66 12 9
0.75
12 8
0.66 12 7 0.58
0.08
−0.08
10
c
12 6 0.50 12 8
0.66
12 6
0.50 12 5 0.42
0.08
−0.16
11 NA NA NA NA NA
NA NA NA NA NA NA NA
NA
NA
12 12 7 0.58 12 7
0.58
12 7
0.58 12 4 0.42
0.16
0.00
13 12 5 0.42 12 5
0.42
12 5
0.42 12 6 0.50
−0.08
0.00
MPN, CFU/
test portion
Estimate 0.66
All
132 86 0.65 132 86
0.65 132 85 0.64 132 64 0.48
0.16
0.00
LCL
0.51
0.57
0.57
0.56
0.40
0.04
−0.12
UCL
0.83
0.73
0.73
0.73
0.57
0.28
0.12