86 / 199
774
B
ird
et al
.
:
J
ournal of
AOAC I
nternational
V
ol
. 98, N
o
. 3, 2015
Table 2. Total yeast and mold log
10
counts for frozen raw ground beef patties by 3M Petrifilm RYM Count Plate after 48 h incubation at 25
°
C versus FDA BAM (ref. 1) and ISO
21527 (ref. 4) reference methods
a
Uninoculated control
Low
Medium
High
Petrifilm RYM FDA BAM/ISO 21527
b
Petrifilm RYM FDA BAM/ISO 21527
Petrifilm RYM
FDA BAM/ISO 21527
Petrifilm RYM FDA BAM/ISO 21527
Lab
A
c
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
1
<1.00
<1.00
<1.00
<1.00
2.18
1.78
2.23
1.60
3.45
3.38
3.49
3.32
4.64
4.62
4.32
4.54
2
<1.00
<1.00
<1.00
<1.00
2.43
2.48
2.43
2.83
3.62
3.71
3.49
3.45
4.62
4.73
4.53
4.52
3
<1.00
<1.00
<1.00
<1.00
2.34
2.30
2.41
2.32
3.52
3.30
3.63
3.53
4.64
4.40
4.82
4.81
4
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5
<1.00
<1.00
<1.00
<1.00
1.95
1.78
1.70
2.00
3.41
3.53
3.30
3.41
4.43
4.51
4.46
4.64
6
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
7
<1.00
<1.00
<1.00
<1.00
2.23
2.36
1.80
1.82
3.51
3.56
3.36
3.61
4.79
4.32
4.43
4.49
8
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
9
<1.00
<1.00
<1.00
<1.00
2.45
2.23
2.28
2.23
3.56
3.60
3.51
3.45
4.72
4.73
4.57
4.79
10
<1.00
<1.00
<1.00
<1.00
2.20
2.20
2.11
2.00
3.60
3.45
3.45
3.43
4.76
4.61
4.66
4.40
11
<1.00
<1.00
<1.00
<1.00
2.36
1.00
2.40
1.90
3.57
3.66
3.49
3.54
4.68
4.56
4.49
4.59
12
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
13
<1.00
<1.00
<1.00
<1.00
2.45
2.08
2.00
1.67
3.49
3.51
3.52
3.48
4.75
4.89
4.67
4.77
14
<1.00
<1.00
<1.00
<1.00
2.20
1.00
2.34
e
1.00
e
3.41
3.67
3.57
3.70
4.70
4.60
4.65
4.62
15
<1.00
<1.00
<1.00
<1.00
2.30
2.28
2.15
2.23
3.38
3.53
3.38
3.23
4.72
4.85
4.61
4.66
a
Log
10
yeast and mold CFU/g.
b
FDA BAM and ISO 21257 reference method replicates were enumerated at 5 and 7 days (uninoculated test portions) of incubation at 25
°
C
c
A and B = Indicated duplicate test portions.
d
Laboratory data not included in statistical analysis for all contamination levels.
e
Cochran’s outlier.