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Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals
Jaudzems, Lahrichi, and Fuerer
–
January 2017
page 9/20
MW
AA
:
Molecular weight of individual amino acids in g/mol (see below)
V
s
:
Volume of hydrolysis solution in mL (typically 5 mL)
d
1
:
Dilution factor in the neutralization step (4)
d
2
:
Dilution factor in the derivatization step (10)
m
s
:
mass of the test portion in mg
10:
Combined factor to convert pg to mg (10
-9
), mL to µL (10
3
) and mg to 100 g
(1/10
-5
)
Note: Empower can be configured to calculate the mass fraction w
Molecular Weights (
MW
AA
) of Amino Acids (g/mol)
Aspartic acid:
133.11
Methionine:
149.21
Threonine:
119.12
Isoleucine:
131.18
Serine:
105.09
Leucine:
131.18
Glutamic acid:
147.13
Tyrosine:
181.19
Proline:
115.13
Phenylalanine:
165.19
Glycine:
75.07
Lysine:
146.19
Alanine:
89.10
Histidine:
155.16
Cystine:
240.30
Arginine:
174.20
Valine:
117.15
Taurine:
125.15
4
PERFORMANCE CHARACTERISTICS
4.1
Method Performance Requirements (SMPR)
According to their concentration in reconstituted powders or ready-to-feed liquids, analytes
are categorized into three groups: 0.4-5.0, 5.0-50, and 50-2500 mg/100 g reconstituted
product. Table 2 from AOAC SMPR 2014.013 indicates the different method performance
requirements for each of the three groups (see below).
AMINO-01 (FEBRUARY 2017)
SLV REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE