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Method Performance Feedback 2014.03
Are there any reference materials considered or addressed?
Yes
NIST egg powder, NIST non-fat milk powder
No
The study did not use any reference materials because any not available at that time. It did
use FAPAS Proficiency Test samples 2781 and 2792 and 2795.
No
I would be interested to see any available PT data on this method at time of final action.
No
For gluten methods we see that single lab studies, collaborative studies, scientific papers and
preparation of spiked samples refer to the use of PWG gliadin. Sometimes wheat flour
calibrated against PWG gliadin. This in lack of a CRM, but also still part of this ERP's ongoing
discussions
Yes
Wheat gluten calibrator was calibrated against the commercially available WGPAT gliadin
(86% highly purified gliadin from 40 different European wheat variety. However, this is not
the only wheat reference material available.
Are the approved validation protocols available and documented?
Yes
No
Yes
Yes
Yes
Is the method documentation available that demonstrates Reproducibility/Uncertainty and Probability of
Detection? If so, please cite the reference.
STATISTICAL REVIEW: Is the documentation for the statistical review prior to AOAC First Action Official
Methods status available and completed?
Yes
Statistics review completed at the time of adoption as First Action method
n/a - performed at 1st action stage.
?
Currently there is no SMPR for gluten ELISA available. Statistic were done according to App M.