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Exchange ofthimerosal
Page 7
3.3 RIDASCREEN
®
GliadinR7001 (OMA first action 2012.01)
3.3.1
Results
The original raw data (obtained OD values for the standards and control samples) are presented in
the annex section 7.3. From these data, the concentrations of the controls samples were calculated
for each measurement using thimerosal or bronidox L containing wash buffer as shown in table 5.
Table 5
: calculated concentrations of the control samples in mg/kg gliadin.
Stored
buffer
Start
Storage as 10 fold concentrate
Storage as finally diluted
Duration of
storage
0
weeks
3 weeks
6 weeks
3 weeks
6 weeks
Storage
temperature
NA
4°C
37°C
4°C
37°C
4°C
37°C
4°C
37°C
Thimerosal
Control 1
5.17
6.12
5.67
5.32
5.45
6.00
5.89
7.60
7.69
Control 2
12.61
9.00
8.38
7.59
7.25
8.63
8.37
9.30
9.15
Bronidox L
Control 1
5.74
6.11
6.48
6.51
6.84
5.77
5.78
7.08
7.40
Control 2
11.41
7.80
7.86
7.69
7.74
6.59
7.28
8.55
8.80
3.3.2
Evaluation
The obtained values for Gliadin were evaluated in the same way as the obtained values for FAST
Milk, see section 3.1.2. The results are shown in table 6.
Table 6:
calculated mean concentrations and standard deviations (SD) of the control samples in
mg/kg gliadin.
Stored
buffer
Thimerosal
Bronidox L
Storage
temperature
4°C
37°C
4°C
37°C
Mean
SD
Mean
SD
Mean
SD
Mean
SD
Control 1
6.3
0.96
6.2
1.03
6.4
0.56
6.6
0.68
Control 2
8.6
0.75
8.3
0.78
7.7
0.81
7.9
0.64