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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
2
Data are well organized and clearly presented
Method Clarity:
Method is clearly written.
Method lists all the necessary supplies and analysis steps
Method Safety Concerns:
No major safety concerns are noted
Pros/Strengths:
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Uses established methodology and commonly available equipment
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Fast chromatographic analysis
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Method describes alternative eluants and reagents preparations procedures – from
commercially available kits as well as “from scratch” using common chemicals
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Data for all SPIFAN matrices are presented
•
Linearity and other parameters are evaluated and compared with SMPR
Cons/Weaknesses
Accuracy data and intermediate reproducibility data for many amino acids don’t meet SMPR
Method is not suitable for analysis if Tryptophan
Supporting Data
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Method Optimization:
Some method optimization may be needed if baseline separation for amino acids is not
achieved using chromatographic conditions described in the method
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Performance Characteristics:
Analytical Range and Linearity: