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General Analytical Needs
Method should
▪
Separate and accurately determine both vitamin K1 (phylloquinone) and K2 (different
menaquinones)
▪
Determination of
trans
-K1 and
cis
-K1 (defined as the sum of
cis
and
trans
isomer of K1)
▪
Separate and accurately determine three different forms of K2 (MK4, MK6 and MK7)
▪
Determination of all-
trans
-MK4, all-
trans
MK6 and all-
trans
MK7. Many cis forms maybe
present.
▪
Be able to analyze both coated and non-coated formulations
▪
Determine the above in raw materials used to produce/formulate dietary supplements
Challenges
▪
Need cost effective, quick turnaround analysis time and one test method that can
be run in an in-house laboratory.
▪
Vitamin K is unstable to light, alkali and oxidizing agents
▪
Must be protected from light
▪
Use of non-UV lamps
▪
Brown glassware