WORKING DRAFT
ISO/WD
© ISO 2013 – All rights reserved
1
Milk Products and infant formulae — Determination of Labelled
Fatty Acids content — Capillary gas chromatographic method
1 Scope
This International Standard specifies a single method for the quantification of all labelled fatty acids that includes
groups of fatty acids (i.e. TFA, CLA, SFA, MUFA, PUFA, omega-3, omega-6, omega-9) and/or individual fatty
acids (i.e. LA, ALA, ARA, EPA, DHA) (see also Annex A) in milk products, infant formulae and adult/pediatric
nutritionals (i.e. all forms made from any combination of milk, soy, rice, whey, hydrolysed protein, starch and
amino acids, with and without intact protein) containing milk fat and/or vegetable oils, supplemented or not
supplemented with long chain polyunsaturated fatty acids (LC-PUFA).
The determination is performed by direct transesterification of food matrices, without prior fat extraction, and
consequently it is applicable to liquid samples or reconstituted powders.
Products containing less than 1,5 % fat can be analysed after preliminary fat extraction using methods described
at section 2. Dairy products, like soft or hard cheeses with FFA level ≤ 1 mmol/100g of fat can be analysed after
preliminary fat extraction using methods described in section 2.
Consider particular attention in presence of products supplemented/enriched with PUFA having fish oil or algae
origins. In that case, cold fat extraction procedure is recommended (evaporation of solvents at lower
temperature).
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated references,
only the edition cited applies. For undated references, the latest edition of the referenced document (including any
amendments) applies.
ISO 1211 IDF 1,
Milk - Determination of fat content - Gravimetric method (Reference method)
ISO 8381 | IDF123,
Milk-based infant foods - Determination of fat content
ISO 8262-1 | IDF124-1,
Milk products and milk-based foods - Determination of fat content by the Weibull-Berntrop
gravimetric method (Reference method): Part 1: Infant Foods
ISO 1737 | IDF 13,
Evaporated milk & sweetened condensed milk -Determination of fat content
ISO 1740 | IDF 6,
Milk fat products and butter – Determination of fat acidity (Reference method)
ISO 14156 | IDF 172,
Milk and Milk Products – Extraction methods for lipids and liposoluble compounds
3 Terms and definitions
For the purposes of this document, the following term and definition applies.
3.1 fatty acids content
Mass fraction of individual or groups substances determined by the procedure specified in this
International Standard (see Table 1)
NOTE
The fatty acids content is expressed as a mass fraction in grams (or in milligrams) of the fatty acids listed in Table
1 per 100 g of product. Fatty acids results expressed in g (or mg) / 100g product can be converted in other results expression
format (e.g., g / 100 g serving size, g / 100 g reconstituted product for powders).
