ISO/WD
4
© ISO 2013 – All rights reserved
Pentadecanoic acid methyl ester (C15:0), cis-10-Pentadecenoic acid methyl ester (C15:1 n-5 or Δ10 cis), Palmitic
acid methyl ester (C16:0), Palmitoleic acid methyl ester (C16:1 n-7 or Δ9 cis), Heptadecanoic acid methyl ester
(C17:0), cis-10-Heptadecenoic acid methyl ester (C17:1 n-7 or Δ10 cis), Stearic acid methyl ester (C18:0), Elaidic
acid methyl ester (C18:1 n-9 or Δ9 trans), Oleic acid methyl ester (C18:1 n-9 or Δ9 cis), Linolelaidic acid methyl
ester (C18:2 n-6 or Δ9,12 trans), Linoleic acid methyl ester (C18:2 n-6 or Δ9,12 cis), Arachidic acid methyl ester
(C20:0), gamma-Linoleic acid methyl ester (C18:3 n-6 gamma or Δ9,12 cis), cis-11-Eicosenoic acid methyl ester
(C20:1 n-9 cis or Δ11 cis), Linolenic acid methyl ester (C18:3 n-3 cis or Δ9,12,15 cis), Heneicosanoic acid methyl
ester (C21:0), cis-11,14-Eicosadienoic acid methyl ester (C20:2 n-6 cis or Δ11,14 cis), Behenic acid methyl ester
(C22:0), cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3 n-6 cis or Δ8,11,14 cis), Erucic acid methyl ester
(C22:1 n-9 cis or Δ13 cis), cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3 n-3 cis or Δ11,14,17 cis),
Arachidonic acid methyl ester (C20:4 n-6 cis or Δ5,8,11,14 cis), cis-13,16-Docosadienoic acid methyl ester (C22:2
n-6 cis or Δ13,16 cis), Lignoceric acid methyl ester (C24:0), cis-5,8,11,14,17-Eicosapentanoic acid methyl ester
(C20:5 n-3 cis or Δ5,8,11,14,17 cis), Nervonic acid methyl ester (C24:1 n-9 cis or Δ15 cis), cis-4,7,10,13,16,19-
Docosahexaenoic acid methyl ester (C22:6 n-3 cis or Δ4,7,10,13,16,19 cis).
NOTE
The purchasing of individual FAME standards is more expensive in comparison to FAME standard mixture. In
addition the individual weight for each FAME standards could give imprecision.
b) Purchase a quantitative FAME standard mixture: Nu-Check-Prep, Cat. Number GLC- Nestle-36
2)
.
The FAME calibration standard mixture is carefully prepared by weight by the supplier. The weight percentage of
each component is indicated in the accompanying certificate. Each ampoule contains approximately 100 mg of
the FAME calibration standard mix. All individual FAME are distributed in equal proportions in the standard,
excepted for Palmitic acid methyl ester (C16:0) in double amount.
5.19 Home made calibration standard FAME mixture solution
5.19.1 Stock solution 1 - Saturated
Into a 100 mL accurately weigh ca. 25 mg of Lignoceric acid methyl ester (C24:0), 25 mg of Behenic acid methyl
ester (C22:0), 25 mg of Heneicosanoic acid methyl ester (C21:0), 25 mg of Arachidic acid methyl ester (C20:0),
25 mg of Stearic acid methyl ester (C18:0), 25 mg of Heptadecanoic acid methyl ester (C17:0), 50 mg of Palmitic
acid methyl ester (C16:0), 25 mg of Pentadecanoic acid methyl ester (C15:0), 25 mg of Myristic acid methyl ester
(C14:0), 25 mg of Tridecanoic acid methyl ester (C13:0), 25 mg of Lauric acid methyl ester (C12:0), 25 mg of
Undecanoic acid methyl ester (C11:0), 25 mg of Capric acid methyl ester (C10:0), 25 mg of Caprylic acid methyl
ester (C8:0), 25 mg of Caproic acid methyl ester (C6:0) and 25 mg Butyric acid methyl ester (C4:0). Make up to
the mark with n-hexane.
NOTE Palmitic acid is weighed in double amount
5.19.2 Stock solution 2 - Monounsaturated
Into a 100 mL accurately weigh ca. 25 mg of Nervonic acid methyl ester (C24:1 n-9 cis or Δ16 cis), 25 mg of
Erucic acid methyl ester (C22:1 n-9 cis or Δ13 cis), 25 mg of cis-11-Eicosenoic acid methyl ester (C20:1 n-9 cis or
Δ11 cis), 25 mg of Oleic acid methyl ester (C18:1 n-9 cis or Δ9 cis), 25 mg of Elaidic acid methyl ester (C18:1 n-9
trans or Δ9 trans), 25 mg of cis-10-Heptadecenoic acid methyl ester (C17:1 n-7 cis or Δ10 cis), 25 mg of
Palmitoleic acid methyl ester (C16:1 n-7 cis or Δ9 cis), 25 mg of cis-10-Pentadecenoic acid methyl ester (C15:1 n-
5 cis or Δ10 cis) and 25 mg of Myristoleic acid methyl ester (C14:1 n-5 cis or Δ9 cis). Make up to the mark with n-
hexane.
2)
Nu-Check-Prep GLC-Nestle36 is an example of suitable product available commercially This information
is given for the convenience of the user of this International Standard and it does not constitute an
endorsement by either ISO or IDF of this product.
