ISO/WD
© ISO 2013 – All rights reserved
3
5.12 C11:0 FAME/C13:0 TAG standard solution
Into a 250 ml volumetric flask, weigh to the nearest 0,1 mg about 500 mg of tritridecanoin and 500 mg of methyl
undecanoate. Dissolve and make up to the mark with MTBE.
Stored in the dark at 4 °C this solution is stable for one week. Allow the solution to come to room temperature
before use.
This solution volume is sufficient to analyze approx. 40 samples or more. In case or smaller number of analysis,
standard weights and volume of solvent can be adapted accordingly.
5.13 Octadecenoic acid methyl ester
, cis/
trans
-isomers mixture of C18:1 with
trans
-4 to
trans
-16 (all isomers)
and principal cis isomers. Concentration 2,5 mg/ml in Methylene Chloride.
NOTE
This standard is commercially available from Sigma-Aldrich (Cat. 40495-U).
5.14 Linoleic acid methyl ester
,
cis
/
trans
-isomers mixture of C18:2 n-6 with
trans
-9,
trans
-12-octadecadienoic
acid (50%),
cis
-9,
trans
-12-octadecadienoic acid (20%),
trans
-9,
cis
-12-octadecadienoic acid (20%) and
cis
-9,
cis
-
12-octadecadienoic acid (10%). Concentration 10 mg/mL in Methylene Chloride.
NOTE Standard commercially only available from Supelco Inc (Cat. 47791) 1) .
5.15 Linolenic acid methyl ester
,
cis
/
trans
isomer mixture of C18:3 n-6 with
cis
-9,
cis
-12,
cis
-15-
Octadecatrienoic acid methyl ester ~ 3 % (w/w),
cis
-9,
cis
-12,
trans
-15-Octadecatrienoic acid methyl ester ~ 7 %
(w/w),
cis
-9,
trans
-12,
cis
-15-Octadecatrienoic acid methyl ester ~ 7 % (w/w),
cis
-9,
trans
-12,
trans
-15-
Octadecatrienoic acid methyl ester ~ 15 % (w/w),
trans
-9,
cis
-12,
cis
-15-Octadecatrienoic acid methyl ester ~ 7 %
(w/w),
trans
-9,
cis
-12,
trans
-15-Octadecatrienoic acid methyl ester ~ 15 % (w/w),
trans
-9,
trans
-12,
cis
-15-
Octadecatrienoic acid methyl ester ~ 15 % (w/w) and
trans
-9,
trans
-12,
trans
-15-Octadecatrienoic acid methyl
ester ~ 30 % (w/w). Concentration 10 mg/mL in Methylene Chloride.
NOTE This standard is commercially available from Supelco Inc, (Cat. 47792). This standard contains all trans
isomers but their abundance and ratio are different to those observed oils and fats.
5.16 Methyl octadecadienoate conjugated
acids (mixture of C18:2
cis
-9,
trans
-11 and
cis
-10,
trans
-12), of
purity ≥99%.
5.17 Qualitative
Cis
/
Trans
FAME isomers standard mixture solution
For the RT identification of
cis
/
trans
isomers prepare a qualitative standard solution with the standard listed 5.13
to 5.16. All standards commercially available could be used. Into a 50 ml volumetric flask, add each standard
isomer in equal proportion. Dissolve and make up to the mark with hexane. Dilute accordingly to the type of
injector used.
5.18 Standard FAME mixture solution
a) Purchase individual FAME standard list as follow (of purity ≥99%);
Butyric acid methyl ester (C4:0), Caproic acid methyl ester (C6:0), Caprylic acid methyl ester (C8:0), Capric acid
methyl ester (C10:0), Undecanoic acid methyl ester (C11:0), Lauric acid methyl ester (C12:0), Tridecanoic acid
methyl ester (C13:0), Myristic acid methyl ester (C14:0), Myristoleic acid methyl ester (C14:1 n-5 or Δ9 cis),
1)
Supelco Inc is an example of suitable product available commercially. This information is given for the
convenience of the user of this International Standard and it does not constitute an endorsement by either
ISO or IDF of this product.
