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Identification of Venezuelan Equine
Encephalitis Virus (VEEV)
Parameter
Minimum Performance Requirement
AMIL
5000 genome copies / mL
POI at AMIL within sample
collection buffer
POI at AMIL in an aerosol
environmental matrix
0.95
System False-Negative Rate
using spiked aerosol
environmental matrix
%
System False-Positive Rate
using aerosol environmental
matrix
%
Inclusivity panel purified DNA All inclusivity strains (Table II) must be correctly identified
at 2x the AMIL
†
Exclusivity panel purified DNA All exclusivity strains (Table III and Annex IV; part 2) must
test negative at 10x the AMIL
†
•
[1]
Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at
http://www.eoma.aoac.or9
Identification of Venezuelan Equine
Encephalitis Virus (VEEV)
•
[1]
Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at
http://www.eoma.aoac.orVIRUS
Serotype / Variant
Representative Strain
(s)
VEEV
VEE-IAB
Trinidad Donkey
MF-8
VEE-IC
ICVE93, ICVE95
VEE-IE
IEMX63, IEPA62
VEE-ID
1DPA61, 1DPE98,
IDPE06
Table II: Inclusivity Panel
Inclusivity Panel
10