AOAC ISPAM Stakeholder Panel Meeting Book 3-14-17

Identification of Venezuelan Equine

Encephalitis Virus (VEEV)

Parameter

Minimum Performance Requirement

AMIL

5000 genome copies / mL

POI at AMIL within sample

collection buffer

POI at AMIL in an aerosol

environmental matrix

0.95

System False-Negative Rate

using spiked aerosol

environmental matrix

System False-Positive Rate

using aerosol environmental

matrix

Inclusivity panel purified DNA All inclusivity strains (Table II) must be correctly identified

at 2x the AMIL

†

Exclusivity panel purified DNA All exclusivity strains (Table III and Annex IV; part 2) must

test negative at 10x the AMIL

†

•

[1]

Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at

http://www.eoma.aoac.or

Identification of Venezuelan Equine

Encephalitis Virus (VEEV)

•

[1]

Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at

http://www.eoma.aoac.or

VIRUS

Serotype / Variant

Representative Strain

(s)

VEEV

VEE-IAB

Trinidad Donkey

MF-8

VEE-IC

ICVE93, ICVE95

VEE-IE

IEMX63, IEPA62

VEE-ID

1DPA61, 1DPE98,

IDPE06

Table II: Inclusivity Panel

Inclusivity Panel