56 / 83
Working Document
(MCPD ESTERS & GE)
2
48
Limit of Detection (LOD)
49
The smallest amount or concentration of an analyte that can be estimated with acceptable
50
reliability
. Determined as: LOD = blank mean + 3 standard deviations of ten independent
51
analyses of blank or blank spiked at low level (to be agreed upon by Study Directors) (if
52
there is no detectable blank signal). See reference to Appendix L in section 7. Validation
53
Guidance.
54
55
Limit of Quantitation (LOQ)
56
The minimum concentration or mass of analyte in a given matrix that can be reported as a
57
quantitative
result. Determined as: LOQ = blank mean + 10 standard deviations of ten
58
independent analyses with spiked concentration of blank to be <10% of the estimated LOQ.
59
See reference to Appendix L in section 7. Validation Guidance.
60
61
3-MCPD
62
3-MCPD = 3-monochloropropane-1,2-diol or 3-chloropropane-1,2-diol. CAS NO. 96-24-2.
63
See figure 1 for molecular structure.
64
65
2-MCPD
66
Include definition 2-MCPD = 2-monochloropropane-1,3-diol or 2-chloropropane-1,3-diol.
67
CAS NO. 497-04-1.
68
See figure 2 for molecular structure.
69
70
71
glycidol
72
Include definitionglycidol = 2,3-epoxy-1-propanol. CAS NO. 556-52-5.
73
See figure 3 for molecular structure.
74
75
3-MCPD esters
76
3-MCPD esters (bound 3-
MCPD) =
77
Suggestion: All 3-MCPD derivatives being present as fatty acid esters (mono- and diesters).
[Jk2]
78
79
2-MCPD esters
80
2-MCPD esters (bound 2-
MCPD) =
81
Suggestion: All 2-MCPD derivatives being present as fatty acid esters (mono- and diesters).
[Jk3]
82
83
glycidyl esters
84
Include definition (include general)glycidyl esters (bound glycidol) =
85
Suggestion: All glycidyl derivatives being present as fatty acid esters (monoesters).
[Jk4]
86
87
88
Repeatability
89
Variation arising when all efforts are made to keep conditions constant by using the same
90
instrument and operator, and repeating during a short time period. Expressed as the
91
repeatability standard deviation (SD
r
); or % repeatability relative standard deviation
92
(%RSD
r
).
93
94
Reproducibility
95
The standard deviation or relative standard deviation calculated from among-laboratory
96