Engineering Approaches to Biomolecular Motors

Engineering Approaches to Biomolecular Motors: From in vitro to in vivo Poster Abstracts

25-POS

Board 25

Development of a Tissue-engineered Skin Model for Wound Healing

Saurabh Dixit,

Shree Singh

, Vida Dennis.

Alabama State University, Montgomery, Al, USA.

Biomedical tissue engineering, particularly 3D printing, is rapidly emerging as an alternative

model system in the biomedical field to study the molecular interactions between cells and

biomaterials, drugs and pathogens; genetic and proteomics analyses, transplantation and

understanding transplantation immunology. The synthetic tissues are attractive since they have

potential to generate individualized biomolecules to treat various disease conditions. Our

research has been at the forefront in investigating molecules that potentially could control

inflammatory disorders. We use the similar technology to study the skin tissue regeneration and

wound healing which are regulated by the cytokines and other growth factors. We routinely use

lipopolysaccharide (LPS) as a model molecule to study inflammation in epithelial cells. We have

identified interleukin 10 (IL-10) and silver-polyvinilpirrolidone (Ag/PVP) nanoparticles that

effectively control adverse immune functions following exposure to LPS. We are studying

human keratinocytes to generate 3D tissue-engineered skin in presence of LPS as a model system

to test and understand the molecular mechanisms that mediate development of the skin system.

We have shown that the levels of LPS-induced cytokines [tumor-necrosis factor (TNF),

interleukin (IL)-6 or IL-12p40] are significantly reduced in cells. In addition, IL-10 encapsulated

within the biodegradable nanoparticles, PLGA (poly (lactic-co-glycolic acid) coated with

chitosan or PEGylated poly lactic acid (PLA) markedly modulate the LPS-induced inflammation

in cells. Our system provides the basis to develop the 3D skin model system that will enable

researchers to generate skin tissues for wound healing and various other synthetic biomolecules

ready for use in in vivo systems.