Table of Contents Table of Contents
Previous Page  58 / 79 Next Page
Information
Show Menu
Previous Page 58 / 79 Next Page
Page Background

Engineering Approaches to Biomolecular Motors: From in vitro to in vivo Poster Abstracts

53

29-POS

Board 29

Synthesis and Characterization of the Lawnmower: An Artificial Protein-based, Burnt-

bridges Molecular Motor

Damiano Verardo

1

, Chapin Korosec

2

, Martin J. Zuckermann

2

, Heiner Linke

1

, Nancy R. Forde

2

.

1

Lund University, Lund, Sweden,

2

Simon Fraser University, Vancouver, BC, Canada.

In this work we report progress towards constructing an artificial protein motor dubbed

Lawnmower, based on the burnt-bridges concept and inspired by matrix metalloproteinases

(MMPs), involved in the degradation of collagen fibrils.

The Lawnmower consists of a quantum dot hub (16nm diameter), conjugated to multiple trypsin

protease blades via flexible PEG linker molecules [1]. It is expected to undergo biased motion on

a peptide track through enzymatically cleaving peptides into product and seeking substrate-rich

areas, and will remain processive as long as the individual binding events occur before complete

detachment. To guide experiments, Langevin simulations are carried out to provide insight into

Lawnmower design criteria.

To demonstrate the motor's properties, 2D ensemble experiments on peptide covered,

lightguiding III-V nanowires will be performed. Nanowires are grown via metalorganic vapour

deposition, coated with silicon oxide, and alkyne-silanized in order to allow subsequent click

chemistry functionalization by modified peptides. It was recently observed that light emitted by

molecules close to the surface of these nanowires is waveguided to the tip due to coupling of

optical modes. Nanowires coated with peptides that emit fluorescent photons upon cleavage can

then be used as sensors to assess the speed and processivity of the Lawnmower in two

dimensions.

Following optimization of Lawnmower construction, 1D experiments are planned on peptide-

functionalized DNA, where biased motion of the Lawnmower should be clearly evident. This

DNA-supported peptide lawn has controllable average spacing between peptides, allowing

evaluation of processivity as a function of substrate density. Successful implementation of the

Lawnmower in the above assays would result in the first protein-based artificial molecular

motor.

[1] Kovacic, S.,

et al.

, Design and Construction of the Lawnmower, An Artificial Burnt-Bridges

Motor. IEEE Transactions on NanoBioscience, 14(3), 305–312.