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(e).
Identification
.- Identify the ascorbic acid peak in the chromatograms of the
100
sample solutions by comparison with the retention time and UV spectrum of
101
the corresponding peak in the standard solution.
102
H.
Calculations
103
Calculate the concentration of vitamin C, in mg ascorbic acid/100 g expressed in product “as
104
is” for RTF (ready to feed) products or as “reconstituted powder” for powder samples, as
105
follows:
106
1000 x
x Vm x S
100 x
x V x V I)- (A
C
2
3
1
107
where A = response (height or area) of the ascorbic acid peak obtained for the sample
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solution, m = weight of the test portion, in g (2.0 g of product as is or reconstituted powder),
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V
1
= volume of the test solution (volume used to dissolve the test portion) in mL (10 mL), V
2
110
= volume used in the sample dilution (1.0 mL), V
3
= volume of the final sample dilution (10
111
mL).
112
113
Note: if results expressed in powder sample are needed, use the reconstitution rate to
114
calculate (c *225/25).
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AOAC 2012.22 (FINAL VERSION REVIEW) - JUNE 2016
FOR ERP USE ONLY
DO NOT DISTRIBUTE
AOAC OMB Meeting Book
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