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(e).

Identification

.- Identify the ascorbic acid peak in the chromatograms of the

100

sample solutions by comparison with the retention time and UV spectrum of

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the corresponding peak in the standard solution.

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H.

Calculations

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Calculate the concentration of vitamin C, in mg ascorbic acid/100 g expressed in product “as

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is” for RTF (ready to feed) products or as “reconstituted powder” for powder samples, as

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follows:

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1000 x

x Vm x S

100 x

x V x V I)- (A

C

2

3

1

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where A = response (height or area) of the ascorbic acid peak obtained for the sample

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solution, m = weight of the test portion, in g (2.0 g of product as is or reconstituted powder),

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V

1

= volume of the test solution (volume used to dissolve the test portion) in mL (10 mL), V

2

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= volume used in the sample dilution (1.0 mL), V

3

= volume of the final sample dilution (10

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mL).

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Note: if results expressed in powder sample are needed, use the reconstitution rate to

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calculate (c *225/25).

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AOAC 2012.22 (FINAL VERSION REVIEW) - JUNE 2016

FOR ERP USE ONLY

DO NOT DISTRIBUTE

AOAC OMB Meeting Book

394