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tested in these studies. Furthermore, this real-time assay reduces the automated processing time by

1

about 2 hours compared to the previously-validated DuPont™ BAX® System assays for

Salmonella

,

2

reducing the total time to result by at least 5 hours for most matrices. This reduced time, along with the

3

ease of use and excellent accuracy of the BAX® System, make the test method a strong alternative

4

method for

Salmonella

detection.

5

Conclusion

6

These studies, covering an extensive variety of food and environmental matrices, indicate that this

7

alternative method is sensitive, specific, and rapid in the detection of

Salmonella

. Within the statistical

8

constraints of these studies, no differences were found between the reference culture-based methods

9

and the alternative methods using this test kit. These results indicate broad applicability of the method

10

in allowing uncontaminated food to rapidly be released from a manufacturer’s control, protecting foods

11

from entering commerce when contaminated with

Salmonella

, and also in investigating outbreaks when

12

contamination of foods does occur.

13

Dupont BAX Salmonella Pre- Collaborative Study

For Expert Review Panel Use Only

Do Not Distribute

AOAC INTERNATIONAL