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Table 11. BAX® System vs. Reference Method Results – Dairy Products

Sample Type

Media

MPN

a

Test

Portions

BAX® Method

Reference Method

dPOD

C

e

95% CI

f

X

b

POD

C

c

95% CI

f

X

b

POD

CC

d

95% CI

f

Nonfat dry milk (25 g)

Brilliant Green

Water / BHI

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

1.2 (0.79, 1.8)

20

11

0.55

(0.34, 0.74) 11

0.55

(0.34, 0.74)

0

(-0.14, 0.14)

9.4 (5.7, 16)

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.45, 0.45)

Ice cream (25 g)

LB

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

0.31 (0.17, 0.49)

20

9

0.45

(0.26, 0.66)

6

0.30

(0.15, 0.52)

0.15

(-0.14, 0.41)

1.8 (1.1, 2.7)

5

3

0.60

(0.23, 0.88)

4

0.80

(0.38, 1.0)

-0.20

(-0.62, 0.27)

Ice cream (25 g)

BPW

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

0.31 (0.17, 0.49)

20

6

0.30

(0.15, 0.52)

6

0.30

(0.15, 0.52)

0

(-0.14, 0.14)

1.8 (1.1, 2.7)

5

4

0.80

(0.38, 1.0)

4

0.80

(0.38, 1.0)

0

(-0.45, 0.45)

Ice cream (25 g)

Brilliant Green

Water

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

0.31 (0.17, 0.49)

20

6

0.30

(0.15, 0.52)

6

0.30

(0.15, 0.52)

0

(-0.27, 0.27)

1.8 (1.1, 2.7)

5

5

1.0

(0.57, 1.0)

4

0.80

(0.38, 1.0)

0.20

(-0.27, 0.62)

a

MPN = Most Probable Number based on the POD of reference method test portions using the AOAC MPN calculator (with 95% confidence interval)

2

b

X = Number of positive test portions

3

c

POD

C

=

Confirmed candidate method positive outcomes divided by the total number of trials

4

d

POD

R

= Confirmed reference method positive outcomes divided by the total number of trials

5

e

dPOD

C

= Difference between the candidate method and reference method POD values

6

f

95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

7

8

9

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