AOAC RI ERP Final Action Recommendations

AOAC EXPERT REVIEW PANEL FOR MICROBIOLOGY FOR FOODS AND ENVIRONMENTAL SURFACES

AOAC Expert Review Panel Final Action Feedback

Safety committee approved the method before the collaborative study was initiated. Microbiological

safety precautions are addressed in the OMA manuscript and in the method package insert, both of

which were reviewed and approved by the AOAC reviewers.

REFERENCE MATERIALS

o Document efforts undertaken to locate reference materials. Methods may still progress to Final Action

even if reference materials are not available.

SINGLE-LABORATORY VALIDATION

o Data demonstrating response linearity, accuracy, repeatability, LOD/LOQ, and matrix scope must be

present. Experimental designs to collect this data may vary with the method protocol and the intended

use of the method.

This method was independently validated by AOAC RI, AOAC OMA and AFNOR

involving both single multi-laboratory evaluations and meeting statistical acceptance criteria for each

validation organization.

o Resources can be identified by the AOAC Statistics Committee.

REPRODUCIBILITY/UNCERTAINTY AND PROBABILITY OF DETECTION

o Do you have any information that supports regarding the reproducibility of this method as written? If

so, please specify and submit information.

This method was independently validated by AOAC RI,

AOAC OMA and AFNOR involving both single and multi-laboratory evaluations and meeting the

statistical acceptance criteria for approval from each validation organization.

ADDITIONAL FEEDBACK FROM USERS OF METHOD

o Based on your experience with the method, are there any recommended changes to the AOAC First

Action method as written? no

o Document positive and negative feedback from users of the method during the trial period regarding

the apparatus and reagents, general instructions, enrichment, results and interpretation, confirmation,

etc. see below

o Feedback from users demonstrating method ruggedness should be documented.

o Access to the future availability of vital equipment, reference materials, and supplies.

VIDAS® SPT is a proprietary bioMérieux test kit that is commercially available worldwide. The VIDAS SPT

test is a recombinant phage protein based technology designed for use with the automated

enzyme-linked fluorescent assay VIDAS® or mini VIDAS® instruments for next day detection of

Salmonella

in foods and environmental samples. VIDAS SPT has received independent 3

party

approvals from AOAC RI, AOAC OMA and AFNOR demonstrating reproducibility, repeatability,

robustness and overall reliability in single independent laboratory testing and multi-laboratory

collaborative studies in both the United States and Europe.

AOAC Performance Tested Method: PTM#071101

Page