652 / 1195
VIDAS LMX & LPT Collaborative Study
April 19, 2013
OMA-2013-Apr-XXX
disposal. Disposal of these reagents into sinks with copper or lead
1
plumbing should be followed immediately with large quantities of
2
water to prevent potential hazards.
This kit contains products of animal
3
origin. Certified knowledge of the origin and/or sanitary state of the
4
animals does not totally guarantee the absence of transmissible
5
pathogenic agents. It is therefore recommended that these products be
6
treated as potentially infectious and handled observing the usual safety
7
precautions (do not ingest or inhale).
8
9
10
5.2
Test Kit Components
11
5.2.1
LMX Strips
(60)
12
5.2.2
LMX SPR®
(60) – Interior of SPR®s coated with antibodies directed
13
against
L. monocytogenes
specific antigens.
14
5.2.3
LMX Standard (1 x 6mL)
- Ready-to-use. Purified and inactivated
L.
15
monocytogenes
antigen + preservative + protein stabilizer. The
16
confidence interval is indicated on the MLE card after the following
17
mention: "Standard (S1) RFV Range".
18
5.2.4
LMX Positive Control (1 x 3 mL)
- Purified and inactivated
L.
19
monocytogenes
antigen + preservative + protein stabilizer. The
20
confidence interval is indicated on the MLE card after the following
21
mention: "Control C1 (+) Test Value Range".
22
5.2.5
Negative Control (1 x 6mL)
- TRIS buffered saline (TBS) (150 mmol/L) -
23
Tween pH 7.6 + preservative. The maximum acceptable value for the test
24
is indicated on the MLE card after the following mention: "Control C2 (-)
25
Test Value Range".
26
5.2.6
1 MLE Card (Master Lot Entry)
- Specifications for the factory master
27
calibration data required to calibrate the test: to read the MLE data, please
28
refer to the Operator’s Manual.
29
30
5.3
Reagents
31
5.3.1
Supplement for LMX Broth (LMX SUPP) - (bioMérieux ref.
32
42648)
33
5.3.2
LMX Broth
34
5.3.2.1
3-liter bag (bioMérieux ref. 42649)
35
5.3.2.2
225 mL in a 250 mL bottle (bioMérieux ref. 42647)
36
5.3.2.3
225 mL mini-bag (bioMérieux ref. 42749)
37
5.3.3
LPT Broth
38
5.3.3.1
10 ml tubes (réf. bioMérieux 410845)
39
5.3.3.2
225 mL in a 250 mL bottle (bioMerieux 410848)
40
41
5.4
Selective agar
42
5.4.1
ALOA
43
5.4.2
Oxford Agar (OX)
44
5.4.3
Horse blood overlay agar (HL)
45
DRAFT DOCUMENT
10