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Table 2013.2D (cont’d)

: Comparative Results for the Detection of

Listeria monocytogenes

in Queso Fresco (125 g) by the VIDAS LMXMethod

1

with Confirmation on ALOA vs. AOAC OMA 993.12 in a Collaborative Study (High Level)

2

Statistic

MPN/

Test Portion Laboratory

Candidate

presumptive (CP)

Candidate confirmed

(CC)

Candidate result (C)

Reference method

(R)

C vs. R

N

X

POD(CP)

N

X

POD(CC)

N

X

POD(C) N

X POD(R) dLPOD

(C,R)

dLPOD

(CP,CC)

5.41

3.53

8.30

1

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

2

a

NA NA

NA

NA NA

NA

NA NA

NA

NA NA

NA

NA

NA

3

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

4

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

5

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

6

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

7

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

8

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

9

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

10

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

11

a

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

12

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

13

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

14

12

12

1.00

12

12

1.00

12

12

1.00

12

12

1.00

0.00

0.00

Estimate

All

144 144

1.00

144 144

1.00

144 144

1.00

144 144

1.00

0.00

0.00

LCL

0.97

0.97

0.97

0.97

-0.03

-0.03

UCL

1.00

1.00

1.00

1.00

0.03

0.03

s

r

b

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.16

0.16

0.16

0.16

s

L

c

0.00

0.00

0.00

0.00

LCL

0.00

0.00

0.00

0.00

UCL

0.16

0.16

0.16

0.16

s

R

d

0.00

0.00

0.00

0.00

UCL

0.00

0.00

0.00

0.00

LCL

0.22

0.22

0.22

0.22

P

T

e

1.0000

1.0000

1.0000

1.0000

a

Results were not used in statistical analysis due to laboratory error.

3

c

Repeatability Standard Deviation,

d

Among-Laboratory Standard Deviation,

e

Reproducibility Standard Deviation,

f

P Value =Homogeneity test of laboratory PODs

4

NA = Not Applicable(Data not submitted); LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

5

6

33