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DuPont™ BAX

®

System Real-Time PCR Assay for

Salmonella

: Collaborative Study

DuPont Nutrition & Health

Page 12

Conclusion

1

Within the statistical constraints of these studies, no differences were found between the reference

2

culture-based methods and the alternative BAX® System method. These results indicate that the

3

alternative method can be used to allow uncontaminated food to be released rapidly from a

4

manufacturer’s control and prevents

Salmonella

-contaminated foods from entering commerce.

5

Furthermore, this test method can be a valuable tool for outbreak investigations when food

6

contamination events occur.

7

Collaborator feedback indicated that the method was easy to use and that the clear yes/no results

8

provided by the BAX® System software were appreciated. Time and labor savings were cited as key

9

advantages of the test method over the reference culture methods. No negative feedback regarding the

10

method was provided by any of the collaborators.

11

It is recommended that the BAX® System Real Time

Salmonella

PCR test kit be adopted as Official First

12

Action status for the detection of

Salmonella

in a variety of foods, including raw ground beef, ground

13

beef with soy, beef trim, frankfurters, shrimp, ground turkey, chicken wings, poultry rinse, dried eggs,

14

shell eggs, fresh bagged lettuce, frozen peas, orange juice, cream cheese, nonfat dry milk, ice cream,

15

peanut butter, cocoa, white pepper, infant formula, and dry pet food, and on stainless steel, ceramic

16

tile, and plastic surfaces.

17

FINAL (Version 4)