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1

2

3

Table 5. Ruggedness - Sample boiling time

4

5

Trial 1

Trial 2

Strains

Test

Sample boiling time (min.)

Sample boiling time (min.)

4

5

6

4

5

6

Listeria monocytogenes

ATCC 19115

1

1.15

1.19

1.14

1.03

1.00

0.91

2

1.10

1.13

1.23

0.99

0.97

0.95

3

1.17

1.14

1.09

1.08

0.96

0.98

4

1.14

1.14

0.86

1.09

0.94

0.96

5

1.17

1.14

1.13

1.08

0.96

0.96

Mean

1.15

1.15

1.09

1.05

0.97

0.95

Listeria monocytogenes

ATCC 19117

1

1.02

0.99

0.89

0.78

0.76

0.75

2

1.04

0.96

0.85

0.83

0.79

0.72

3

1.03

0.93

0.91

0.84

0.79

0.73

4

1.04

0.94

0.98

0.81

0.76

0.74

5

0.97

0.97

0.90

0.83

0.76

0.70

Mean

1.02

0.96

0.91

0.82

0.77

0.73

Bacillus cereus

ATCC 27522

1

0.00

0.00

0.00

0.00

0.00

0.00

2

0.00

0.00

0.00

0.00

0.00

0.00

3

0.00

0.00

0.00

0.00

0.00

0.00

4

0.00

0.00

0.00

0.00

0.00

0.00

5

0.00

0.00

0.00

0.00

0.00

0.00

Mean

0.00

0.00

0.00

0.00

0.00

0.00

Positive results = TV

0.05, negative results = TV< 0.05

6

ATCC = American Type Culture Collection

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8

9

PTM Certification Report