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DRAFT AOAC SMPR 2016.XXX; Version 4; November 19, 2015
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SMPR Name:
Quantitative measurement of β-cryptoxanthin, lutein, and
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zeaxanthin in ingredients and dietary supplements.
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Intended Use
: Reference method for cGMP compliance.
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Purpose
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AOAC SMPRs describe the minimum recommended performance characteristics to be used during
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the evaluation of a method. The evaluation may be an on-site verification, a single-laboratory
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validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC Stakeholder
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Panels composed of representatives from the industry, regulatory organizations, contract
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laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC
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Expert Review Panels in their evaluation of validation study data for method being considered for
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Performance Tested Methods
or AOAC
Official Methods of Analysis
, and can be used as acceptance
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criteria for verification at user laboratories. [Refer to Appendix F:
Guidelines for Standard Method
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Performance Requirements
,
Official Methods of Analysis of AOAC INTERNATIONAL
(2012) 19th Ed.,
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AOAC INTERNATIONAL, Gaithersburg, MD, USA.]
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Applicability
:
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Separate quantitative determination of β-cryptoxanthin, lutein, and zeaxanthin in ingredients and
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dietary supplements.
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Analytical Technique
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Any analytical technique(s) that measures the analytes of interest and meets the following method
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performance requirements is/are acceptable.
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Definitions
:
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Analytes
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β-Cryptoxanthin
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IUPAC name: (R)-3,5,5-Trimethyl-4-[3,7,12,16-tetramethyl-18-(2,6,6-trimethylcyclohex-1-enyl)-
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octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]-cyclohex-3-enol. CAS registry number: 472-70-8. See
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figure 1 for chemical structure.
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Lutein
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IUPAC name: β,ε-carotene-3,3'-diol. CAS registry number 1 27-40-2. See figure 2 for chemical
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structure.
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Zeaxanthin
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IUPAC name: 4-[18-(4-hydroxy-2,6,6-trimethyl-1-cyclohexenyl)-3,7,12,16-tetramethyl-octadeca-
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1,3,5,7,9,11,13,15,17-nonaenyl]-3,5,5-trimethyl-cyclohex-3-en-1-ol. CAS registry number: 144-
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68-3. See figure 3 for chemical structure.
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