AOAC SPIFAN Review Team Meeting Book-July 22, 2015

Silliker

Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

Validation of A LC-MS/MS Method for Folate Analysis in i

nfant Formula

and Adult Nutritional Samples

Objective:

Develop and validate a LC-MS/MS method for quantitation of folate in infant formula and adult

nutritional samples

Analyte Scope:

Folic acid (FA), , 5-methyl-5,6.7,8-tetrahydrofolate (5-CH3-THF), 5-formyl-5,6.7,8-tetrahydrofolate

(5-CHO-THF), 5,6.7,8-tetrahydrofolate (THF), 5,10 methenyl--5,6.7,8-tetrahydrofolate (5,10-CH=CH-

THF), 10-methyl-folic acid (CH3-FA), 10-formyl-folic acid (CHO-FA).

Matrix Scope:

Infant Formula Samples

Adult Nutritional

SPIFAN Samples Used for the Method Validation in this study

Provided to Silliker by SPIFAN on 3/26/14

1.)

Child Formula Powder

Lot # 00411RF00

2.)

Infant Elemental Powder

Lot # 00402RF00

3.)

Adult Nutritional RTF, High Protein

Lot # 00415RF00

4.)

Adult Nutritional RTF, High Fat

Lot # 00407RF00

5.)

Infant Formula RTF, Milk Based

SPIFAN Blank Milk Form

The above blank samples are used for the evaluation of spike recovery of folates by the method

6.)

SRM 1849A

Lot # CLC10-b

7.)

Adult Nutritional Powder Milk Protein Based

Lot # 11750017V3

8.)

Infant Formula Powder Partially Hydrolyzed Milk Based

2011.06 (Fol-22) w/SLV

FOR ERP USE ONLY

DO NOT DISTRIBUTE