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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
5
Tetrahydrofolic acid, calcium salt
6.) 10-Methylfolic acid
Schircks Laboratories
Cat. No. 16.211
7.) (6R,S)-5,10-Methylene-5,6,7,8-
Schircks Laboratories
Cat. No. 16.226
Tetrahydrofolic acid, calcium salt
8.) 10-Formylfolic acid
Schircks Laboratories
Cat. No. 16.215
9.) Pteroyltri-y-L-glutamic acid Schircks Laboratories
Cat. No. 16.253
(Folic acid, triglutamate)
10.) (6R,S)-5(Formyl-13C)-5,6,7,8- Schircks Laboratories
Cat. No. 9622
Tetrahydrofolic acid, lithium salt
11.) (5-15N)-Folic Acid
Schircks Laboratories
Cat. No. 96.203
12.)
13
C-Folic Acid (Internal Standard) Merck
Cat. No. CH2QC 900824
13)
13
C5-labeled (6S)-5-methyltetrahydrofolic acid, calcium salt (Internal Std)
((6S)-5-CH3-H4Pte[13C5]Glu-Ca) Merck Cat No. CH2QC 900827
14.) a-amylase
Sigma-Aldrich
Cat. No. A6211
15.) protease from bacillus
Sigma-Aldrich
Cat. No. P3910
16.) Conjugase (Rat Plasma)
Bioreclamation LLC
Cat. No. RATPLLIHP-M
17.) Ammonium Hydroxide Fisher
Cat. No. A669-500
18.) Sodium Phosphate, dibasic salt Fisher
Cat. No. S-374-500
19.) Methanol, Optima LC/MS, 99.9% Fisher
Cat. No. A456-4
20.) Acetic Acid
Fisher
Cat. No. A38S-212
21.) Sodium Hydroxide
Fisher
Cat. No. S318-1
22.) Ammonium Formate
Sigma-Aldrich
Cat. No. 17843-250G
23.) Ammonium Acetate
Fisher
Cat. No. AX1220-500G
24.) 2-Mercaptoethanol
Fisher
Cat. No. BP176-100
25.) Ascorbic Acid
Fisher
Cat. No. BP351-500
26.) Water, Optima
Fisher
Cat. No. W7-4
27.) TCEP
Fisher
Cat. No. 50-093-0
28.) Charcoal
Fisher
Cat. No. D127-500
29) Ammonium hydroxide Fisher Cat. No. A669-500
(Ammonia soln. 28.87%)
Apparatus:
1.)
Shimadzu UFLC system
2.) API 3200 Mass Spectrometer
3.) Mettler Toledo pH Meter
4.) 24-well SPE Vacuum manifold
5.) Refrigerator (capable of cooling to 4
o
C)
6.) Freezer (capable of cooling to -20
o
C)
7.) Water Bath, with shaker
8.) Centrifuge (capable of up to 2000rpm)
9.) Microfuge (capable of up to 15000rpm)
Supplies:
1.) Disposable Glass Culture Tubes Fisher
Cat. No. 14-961-30
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE