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b)

Catalog Number

.

MDA2LIS96

1

2

Test Kit Reagents -

3M MDA 2 -

Listeria

(96 tests)

3

a)

Lysis Solution (LS) tubes

. – 96 (12 strips of 8 tubes)

4

b)

Listeria Reagent tubes

. – 96 (12 strips of 8 tubes)

5

c)

Extra caps.

– 96 (12 strips of 8 caps)

6

d)

Reagent Control (RC).

– 16 (2 pouches of 8)

7

e)

Quick Start Guide

8

9

Additional Supplies and Reagents

10

a)

3M Molecular Detection System (instrument), power supply, cord, USB cable and

11

software

12

b)

3M Molecular Detection Speed Loader Tray

13

c)

3M Molecular Detection Chill Block insert

14

d)

3M Molecular Detection Heat Block Insert

15

e)

3M Molecular Detection Cap/Decap Tool [Reagent]

16

f)

3M Molecular Detection Cap/Decap Tool [Lysis]

17

g)

Empty lysis tube rack

18

h)

Empty reagent tube rack

19

i)

Dey-Engley (D/E) Neutralizing Broth

20

j)

Demi Fraser Broth (with the addition of Ferric Ammonium Citrate [FAC])

21

k)

3M™ Hydrated Sponge Stick with 10 mL of D/E (3M Center St. Paul, MN)

22

l)

3M™ Molecular Detection Matrix Control Kit (3M Center St. Paul, MN)

23

24

Additional media

25

b)

Oxford Agar (OX)

26

c)

27

d)

Fraser Broth (FB)

28

e)

Sheep Blood Agar (SBA)

29

f)

PALCAM Agar

30

g)

Ottaviani Agosti Agar (OAA)

31

h)

Trypticase Soy Agar (TSA)

32

i)

Trypticase Soy Agar with 0.6% Yeast Extract (TSA/YE)

33

j)

De Man, Rogosa and Sharpe (MRS)

34

k)

Modified Oxford Agar (MOX)

35

l)

Brain Heart Infusion (BHI) broth

36

m)

Horse Blood Overlay (HBO)

37

n)

Buffered Listeria Enrichment Broth (BLEB)

38

39

Apparatus

40

a)

Pipettes

. – capable of 20µL

41

b)

Multi-channel pipette

. – capable of 20µL

42

c)

Sterile pipette tips.

– capable of 20µL

43

d)

Stomacher®

. – Seward or equivalent

44

e)

Filter Stomacher® bags

. - Seward or equivalent

45

f)

Thermometer

. – calibrated range to include 100 ± 1°C range

46

g)

Incubators

. – capable of maintaining 37 ± 1°C

47

AOAC Research Institute

Exp rt Review Panel Use Only

OMAMAN-29 D/ PTM Validation Report 111501

OMA ERP - June 2016

ERP Use Only