196-197 / 596
Table 8: 3M
™
MDA 2 -
Listeria
Assay, Candidate vs. Reference – POD Results (INTERNAL STUDY)
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
USDA/FSIS MLG
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Raw Chicken
Leg Pieces
h
(25 g)
L. ivanovii
Ad 1291
28
Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.31 (0.14, 0.57)
20
4
0.20
0.08, 0.42
5
0.25
0.11, 0.47
-0.05
-0.30, 0.21
0.5
5
3
0.60
0.23, 0.88
1
0.20
0.04, 0.62
0.40
-0.16, 0.73
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
USDA/FSIS MLG
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Raw Chicken
Fillet
i
(25 g)
L. ivanovii
Ad 1291
28Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.3 (0.10, 0.5)
20
4
0.20
0.08, 0.42
5
0.25
0.11, 0.47
-0.05
-0.30, 0.21
0.5
5
2
0.40
0.12, 0.77
4
0.80
0.38, 0.96
-0.40
-0.73, 0.16
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
FDA-BAM
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Bagged Raw
Spinach
h
(25 g)
L. seeligeri
Ad 1754
24
Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.26 (0.10, 0.47)
20
6
0.30
0.15, 0.52
3
0.15
0.05, 0.36
0.15
-0.11, 0.39
1.7
5
3
0.60
0.23, 0.88
4
0.80
0.38, 0.96
-0.20
-0.60, 0.31
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
ISO 11290-1
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Bagged Raw
Spinach
h
(25 g)
L. seeligeri
Ad 1754
24
Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.26 (0.10, 0.47)
20
6
0.30
0.15, 0.52
9
0.45
0.26, 0.66
-0.15
-0.41, 0.14
1.7
5
3
0.60
0.23, 0.88
4
0.80
0.38, 0.96
-0.20
-0.60, 0.31
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
FDA-BAM
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Cold Smoked
Salmon
h
(25 g)
L. innocua
Ad 1674
24
Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.27 (0.10, 0.49)
20
10
0.50
0.30, 0.70
7
0.35
0.18, 0.57
0.15
-0.15, 0.41
1.7
5
5
1.00
0.57, 1.00
4
0.80
0.38, 0.96
0.20
-0.26, 0.62
Matrix
Strain
Analysis
Time
Point
MPN
a
/
Test Portion
N
b
3M MDA2
ISO 11290-1
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
X
POD
R
e
95% CI
Cold Smoked
Salmon
h
(25 g)
L. innocua
Ad 1674
24
Hours
-
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
0.27 (0.10, 0.49)
20
10
0.50
0.30, 0.70
8
0.40
0.22, 0.61
0.10
-0.19, 0.37
1.7
5
5
1.00
0.57, 1.00
4
0.80
0.38, 0.96
0.20
-0.26, 0.62
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only