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Table 6: Inoculum Heat Stress Results
Matrix
Test
Portion Size
Inoculating Organism
Agar
CFU/ g
Percent Injury
Beef Hot Dogs
1
25 g
Listeria monocytogenes
ATCC 7644
TSA
1.8 x 10
8
58.3 %
MOX
7.5 x 10
7
Deli Turkey
125 g
Listeria monocytogenes
ATCC 19116
TSA
2.6 x 10
9
68.5 %
MOX
8.2 x 10
8
Queso Fresco
25 g
Listeria monocytogenes
ATCC CWD 1554
TSA
2.6 x 10
9
72.7%
MOX
7.1 x 10
8
Beef Hot Dogs
2
25 g
Listeria monocytogenes
ATCC 7644
TSA
2.6 x 10
9
73.8%
MOX
6.8 x 10
8
1
- Testing for Robustness and Lot to Lot
2
- Matrix Study
TSA: Trypticase soy agar
MOX: modified Oxford agar
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only