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Table 4a: Independent Study - Matrixes and Inoculating Organisms
1
Matrix
Inoculating
Organism
(Culture Condition)
Target Inoculum Level
# of
Replicates
MDA 2
Testing
Time
Points
Reference Method
(Enrichment)
Deli Turkey
L. monocytogenes
ATCC
1
19116
(heat-stressed)
0 CFU/Test Portion
5
24 hours
USDA/FSIS MLG 8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Raw Chicken
Leg Pieces
Naturally
Contaminated
Lot 1
&
Lot 2
20
&
20
28 hours
USDA/FSIS MLG 8.09
(UVM)
Whole Melon
L. monocytogenes
FSL
3
J1-049
0 CFU/Test Portion
5
26 hours
FDA/BAM Chapter 10
(BLEB)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Vanilla Ice
Cream
L. monocytogenes
ATCC
1
19114
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Queso Fresco
L. monocytogenes
CWD
2
1554
(heat-stressed)
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
4% Milk Fat
Cottage Cheese
L. monocytogenes
ATCC
1
19112
&
L. welshimeri
ATCC
1
35897
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Beef Hot Dogs
L. monocytogenes
ATCC
1
7644
(heat-stressed)
0 CFU/Test Portion
5
24 hours
USDA/FSIS MLG 8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Stainless Steel
L. monocytogenes
ATCC1 19118 &
10x Enterococcus
faecium
ATCC 19434
0 CFU/1” x 1” (5 cm2)
5
24 and 26
hours
USDA/FSIS MLG 8.09
(UVM)
~50 CFU/1” x 1” (5
cm2)
20
0 CFU/4” x 4” (100 cm2)
5
Sealed
Concrete
L. monocytogenes
ATCC1 19117
0 CFU/4” x 4” (100 cm2)
5
24 and 26
hours
USDA/FSIS MLG 8.09
(UVM)
~50 CFU/4” x 4” (100
cm2)
20
~100 CFU/4” x 4” (100
cm2)
5
Plastic
L. monocytogenes
ATCC1 51782
&
L. seeligeri
ATCC1 35967
&
0 CFU/1” x 1” (5 cm2)
5
24 and 26
hours
USDA/FSIS MLG 8.09
(UVM)
~50 CFU/1” x 1” (5
cm2)
20
~100 CFU/1” x 1” (5
cm2)
5
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only