280 / 596
a)
3M Molecular Detection System (instrument), power supply, cord, USB cable and
1
software
2
b)
3M Molecular Detection Speed Loader Tray
3
c)
3M Molecular Detection Chill Block Tray and Chill block insert
4
d)
3M Molecular Detection Heat Block Insert
5
e)
3M Molecular Detection Cap/Decap Tool [Reagent]
6
f)
3M Molecular Detection Cap/Decap Tool [Lysis]
7
g)
Empty lysis tube rack
8
h)
Empty reagent tube rack
9
i)
Dey-Engley (D/E) Neutralizing Broth
10
j)
Demi-Fraser broth (with additional of ferric ammonium citrate)
11
12
Additional media
13
a)
Oxford Agar (OX)b) Sheep blood agar (SBA)
14
b)
Fraser broth (FB)
15
c)
Sheep blood agar (SBA)
16
d)
PALCAM agar
17
e)
Ottaviani Agosti Agar (OAA)
18
f)
Trypticase soy agar (TSA)
19
g)
Trypticase soy agar with 0.6% Yeast extract (TSA/YE)
20
h)
De Man, Rogosa and Sharpe (MRS)
21
i)
Modified Oxford agar (MOX)
22
j)
Brain heart infusion (BHI) broth
23
k)
Horse blood overlay (HBO)
24
l)
Buffered Listeria enrichment broth (BLEB)
25
26
Apparatus
27
a)
Pipettes
. – capable of 20µL
28
b)
Multi-channel pipette
. – capable of 20µL
29
c)
Sterile pipette tips.
– capable of 20µL
30
d)
Stomacher®
. – Seward or equivalent
31
e)
Filter Stomacher® bags
. - Seward or equivalent
32
f)
Thermometer
. – calibrated range to include 100 ± 1°C range
33
g)
Incubators
. – capable of maintaining 37 ± 1°C
34
h)
Dry double block heater unit.
– capable of maintaining 100 ± 1°C;
or
a water bath
35
capable of maintaining 100 ± 1°C
36
i)
Freezer
. – capable of maintaining -10 to -20°C, for storing the 3M Molecular Detection
37
Chill Block Tray
38
j)
Refrigerator
. – capable of maintaining 2-8°C, for storing the 3M MDA2
39
k)
Computer
. – compatible with the 3M Molecular Detection System (instrument)
40
41
Safety Precautions
42
43
The user should read, understand and follow all safety information in the instructions for the 3M
44
Molecular Detection System and the 3M Molecular Detection Assay 2 –
Listeriamonocytogenes
.
45
Retain the safety instructions for future reference.
46
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only