352 / 596
14
test portions evaluated by the USDA/FSIS MLG reference method, 60 out of 132 test portions
1
produced positive results. A dLPOD
C
value of 0.04 with 95% confidence intervals of
2
(-0.08, 0.17) was obtained between the candidate and reference method, indicating
the difference
3
between methods was not statistically significant at the 0.05 probability levelno statistical
4
significant difference between the two methods.
A dLPOD
CP
value of 0.02 with 95% confidence
5
intervals of (-0.11, 0.14) was obtained between presumptive and confirmed results indicating
the
6
difference between presumptive and confirmed results was not statistically significant at the 0.05
7
probability
level.nostatistically significant difference between the presumptive and confirmed
8
results.
9
For the high inoculum level, 132 out of 132 test portions (POD
CP
of 1.00) were reported as
10
presumptive positive by the 3M MDA 2 –
Listeria
method with 132out of 132 test portions
11
(POD
CC
of 1.00) confirming positive.For samples that produced presumptive positive results on
12
the 3M MDA 2 –
Listeria
method, 132out of 132 samples confirmed positive (POD
C
of 1.00). For
13
test portions evaluated by the USDA/FSISMLG reference method, 132 out of 132 test portions
14
produced positive results. A dLPOD
C
value of 0.00 with 95% confidence intervals of (-0.03,
15
0.03) was obtained between the candidate and reference method, indicating
the difference
16
between methods was not statistically significant at the 0.05 probability level. no statistical
17
significant difference between the two methods.
A dLPOD
CP
value of 0.00 with 95% confidence
18
intervals of (-0.03, 0.03) was obtained between presumptive and confirmed results indicating
the
19
difference between presumptive and confirmed results was not statistically significant at the 0.05
20
probability
level.nostatistically significant difference between the presumptive and confirmed
21
results.
22
For the un-inoculated controls, 0 out of 132 samples (POD
CP
of 0.00) produced a presumptive
23
positive result by the 3M MDA 2 -
Listeria
method with 0out of 132 test portions (POD
CC
of
24
0.00) confirming positive.For samples that produced presumptive positive results on the 3M
25
MDA 2 –
Listeria
method, 0out of 132 samples confirmed positive (POD
C
of 0.00). For test
26
portions evaluated by the USDA/FSIS MLG reference method, 0 out of 132 test portions
27
produced positive results. A dLPOD
C
value of 0.00 with 95% confidence intervals of (-0.03,
28
0.03) was obtained between the candidate and reference method, indicating
the difference
29
between methods was not statistically significant at the 0.05 probability
level.nostatistical
30
significant difference between the two methods.
A dLPOD
CP
value of 0.00 with 95% confidence
31
intervals of (-0.03, 0.00) was obtained between presumptive and confirmed results indicating
the
32
difference between presumptive and confirmed results was not statistically significant at the 0.05
33
probability
level.nostatistically significant difference between the presumptive and confirmed
34
results.
35
36
Detailed results of the POD statistical analysis are presented in Table 2016.2A and Figures 1A-
37
1B.
38
39
Raw Chicken Breast Fillet (25 g Test Portions)
40
41
Raw chicken breast fillet test portions were inoculated at a low and high inoculum level and were
42
analyzed for the detection of
Listeria
spp. Un-inoculated controls were included in each
43