442 / 596
Table 4b: Internal Study - Matrixes and Inoculating Organisms
1
Matrix
Inoculating
Organism
(Culture Condition)
Target Inoculum Level
# of
Replicates
MDA 2
Testing Time
Points
Reference Method
(Enrichment)
Raw
chicken
2
leg
pieces
L. ivanovii
Ad
1
1291
0 CFU/Test Portion
5
28 hours
USDA/FSIS MLG
8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Raw chicken
fillet
3
L. ivanovii
Ad 1291
0 CFU/Test Portion
5
28 hours
USDA/FSIS MLG
8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Bagged raw
2
spinach
Listeria seeligeri
Ad 1754
0 CFU/Test Portion
5
24 hours
FDA/BAM
Chapter 10
(BLEB)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Bagged
raw
2
spinach
Listeria seeligeri
Ad 1754
0 CFU/Test Portion
5
24 hours
ISO 11290-1/A1
(Half Fraser/Demi
Fraser)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Cold smoked
2
salmon
Listeria innocua
Ad 1674
0 CFU/Test Portion
5
24 hours
FDA/BAM
Chapter 10
(BLEB)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Cold smoked
2
salmon
Listeria innocua
Ad 1674
0 CFU/Test Portion
5
24 hours
ISO 11290-1/A1
(Half Fraser/Demi
Fraser)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
1
Ad – Adria Developpement Culture Collection
2
2
Tested using Demi Fraser brand (with FAC) X
3
3
Tested using Demi Fraser brand (with FAC) Z
4