486 / 596
14
positive (POD
C
of 0.50). For test portions evaluated by the USDA/FSIS MLG reference method,
1
60 out of 132 test portions produced positive results.A dLPOD
C
value of 0.04 with 95%
2
confidence intervals of (-0.08, 0.17) was obtained between the candidate and reference method,
3
indicating
the difference between methods was not statistically significant at the 0.05 probability
4
level.nostatistically significant difference between the two methods.
A dLPOD
CP
value of 0.02
5
with 95% confidence intervals of (-0.10, 0.15) was obtained between presumptive and confirmed
6
results indicating
the difference between presumptive and confirmed methods was not
7
statistically significant at the 0.05 probability
level.nostatistically significant difference between
8
the presumptive and confirmed results.
9
For the high inoculum level, 132 out of 132 test portions (POD
CP
of 1.00) were reported as
10
presumptive positive by the 3M MDA 2 –
Listeria monocytogenes
method with 132out of 132
11
test portions (POD
CC
of 1.00) confirming positive.For samples that produced presumptive
12
positive results on the 3M MDA 2 –
Listeria monocytogenes
method, 132out of 132 samples
13
confirmed positive (POD
C
of 1.00).For test portions evaluated by the USDA/FSISMLG
14
reference method, 132 out of 132 test portions produced positive results. A dLPOD
C
value of
15
0.00 with 95% confidence intervals of (-0.03, 0.03) was obtained between the candidate and
16
reference method, indicating
the difference between methods was not statistically significant at
17
the 0.05 probability
level.nostatistically significant difference between the two methods
.
18
A dLPOD
CP
value of 0.00 with 95% confidence intervals of (-0.03, 0.03) was obtained between
19
presumptive and confirmed results indicating
the difference between presumptive and confirmed
20
methods was not statistically significant at the 0.05 probability
level.nostatistically significant
21
difference between the presumptive and confirmed results.
22
For the un-inoculated controls, 0 out of 132 samples (POD
CP
of 0.00) produced a presumptive
23
positive result by the 3M MDA 2 -
Listeria monocytogenes
method with 0out of 132 test portions
24
(POD
CC
of 0.00) confirming positive. For samples that produced presumptive positive results on
25
the 3M MDA 2 –
Listeria monocytogenes
method, 0out of 132 samples confirmed positive
26
(POD
C
of 0.00). For test portions evaluated by the USDA/FSIS MLG reference method, 0 out of
27
132 test portions produced positive results. A dLPOD
C
value of 0.00 with 95% confidence
28
intervals of (-0.03, 0.03) was obtained between the candidate and reference method, indicating
29
the difference between methods was not statistically significant at the 0.05 probability
level.no30
statistically significant difference between the two methods.
A dLPOD
CP
value of 0.00 with 95%
31
confidence intervals of (-0.03, 0.00) was obtained between presumptive and confirmed results
32
indicating
the difference between presumptive and confirmed methods was not statistically
33
significant at the 0.05 probability
level.nostatistically significant difference between the
34
presumptive and confirmed results.
35
36
Detailed results of the POD statistical analysis are presented in Table 2016.2A and Figures 1A-
37
1B.
38
39
Raw Chicken Breast Fillet (25 g Test Portions)
40
41
Raw chicken breast fillet test portions were inoculated at a low and high inoculum level and were
42
analyzed for the detection of
Listeria monocytogenes
. Un-inoculated controls were included in
43