577 / 596
Table 5b: Internal Study - Aerobic Plate Count and Background Results (Prior to Inoculation)
Matrix
(Test Portion)
APC
1
(CFU/g)
Background
Listeria
Raw Chicken Leg Pieces
(25 g)
5.4 x 10
5
None Detected
Bagged Raw Spinach
(25 g)
8.0 x 10
6
None Detected
Cold Smoked Salmon
(25 g)
4.0 x 10
2
None Detected
1
APC conducted in accordance with FDA/BAM Chapter 3
2
Listeria species
screen conducted following the USDA/FSIS MLG 8.09 guidelines
3
Listeria species
screen conducted following the FDA/BAM Chapter 10 guidelines
4
Listeria species
screen conducted following the ISO 11290-1/1A guidelines
Table 6: Inoculum Heat Stress Results
Matrix
Test Portion
Size
Inoculating Organism
Agar
CFU/ g
Percent Injury
Beef Hot Dogs
1
25 g
Listeria monocytogenes
ATCC 7644
TSA
1.8 x 10
8
58.3 %
MOX
7.5 x 10
7
Deli Turkey
125 g
Listeria monocytogenes
ATCC 19116
TSA
2.6 x 10
9
68.5 %
MOX
8.2 x 10
8
Queso Fresco
25 g
Listeria monocytogenes
ATCC CWD 1554
TSA
2.6 x 10
9
72.7%
MOX
7.1 x 10
8
Beef Hot Dogs
2
25 g
Listeria monocytogenes
ATCC 7644
TSA
2.6 x 10
9
73.8%
MOX
6.8 x 10
8
1
- Testing for Robustness and Lot to Lot
2
- Matrix Study
TSA: Trypticase soy agar
MOX: modified Oxford agar