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Table of Contents 		II
Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler 		3
High-Throughput TruSeq® Stranded Total RNA Library Construction from FFPE Samples on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation 		9
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 		15
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP 		21
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit® on the BiomekFXP Automated Liquid Handler 		29
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler 		35
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler 		39
Nucleic Acid Extraction and Purification Kits 		47
Solid Phase Reverse Immobilization (SPRI) Bead Technology for Micro RNA Clean Up using the Agencourt RNAClean XP Kit. 		53
Agencourt® RNAClean™ XP - SUPPLEMENTAL PROTOCOL FOR Micro RNA CLEAN UP USING AGENCOURT® RNAClean® XP 		57
Micro RNA and Total RNA Purification from Tissues using the Agencourt RNAdvance Tissue Kit.. 		59
Agencourt® RNAdvance™ Tissue Kit - SUPPLEMENTAL PROTOCOL FOR Micro RNA AND TOTAL RNA ISOLATION FROM TISSUE 		63
Micro RNA and Total RNA Purification from Blood Stabilized in PAXgene Blood RNA Tubes using the Agencourt RNAdvance Blood Kit. 		67
Agencourt RNAdvance Blood Kit - Supplemental Protocol for Micro RNA and Total RNA Isolation from PAXgene Preserved Blood 		70
MicroRNA Extraction from Exosomes using Beckman’s Agencourt RNAdvance Cell v2 Kits 		74
Agencourt® RNAdvance® CELL v2 Kit Supplemental Protocol for miRNA and total RNA Isolation from Exosomes 		77
Automation of Micro RNA and Total RNA Purification from Tissues Using the Agencourt RNAdvance Tissue Kits and Biomek Span-8 Liquid Handler 		81
Automation of Micro RNA and Total RNA Purification from Formalin fixed paraffin-embedded tissues (FFPE) using the Agencourt FormaPure Kit and Biomek NXP Span 8 Laboratory Automation Workstation 		85
MicroRNA Extraction from Laser Capture Micro-Dissected (LCM) FFPE Tissue Using Beckman Coulter’s Agencourt FormaPure Kits.. 		89
Highly Efficient MicroRNA and Total RNA Purification from Formalin-fixed paraffin-embedded tissues (FFPE) using Agencourt FormaPure Kit 		91
PCR Reaction Setup Application 		95
PCR Reaction Setup and AMPure XP Application 		99
Fast, Reliable, Automated PCR Reaction Setup 		103
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