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Appendix 3: Limit of detection / quantification

Choline:

Replicate

10,239 ng/g

9,989 ng/g

9,438 ng/g

10,592 ng/g

11,081 ng/g

10,989 ng/g

9,699 ng/g

11,089 ng/g

11,024 ng/g

11,752 ng/g

Mean 10,589 ng/g

Std

0,729 ng/g

LOD 2,2 ng/g

LOQ 7,3 ng/g

In samples

LOD 303 ng/g = 0,3 µg/g

LOQ 1004 ng/g = 1,0 µg/g

Appendix 4: Robustness.

Time Pet food

Milk powder

(hour) Choline (mg/kg) Choline (mg/kg)

1

1945

559

2

2070

570

3

2104

574

4

2134

577

5

2154

577

22

2173

577

Carn-06 (Choline Validation Report)

FOR ERP USE ONLY

DO NOT DISTRIBUTE