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Appendix 3: Limit of detection / quantification
Choline:
Replicate
10,239 ng/g
9,989 ng/g
9,438 ng/g
10,592 ng/g
11,081 ng/g
10,989 ng/g
9,699 ng/g
11,089 ng/g
11,024 ng/g
11,752 ng/g
Mean 10,589 ng/g
Std
0,729 ng/g
LOD 2,2 ng/g
LOQ 7,3 ng/g
In samples
LOD 303 ng/g = 0,3 µg/g
LOQ 1004 ng/g = 1,0 µg/g
Appendix 4: Robustness.
Time Pet food
Milk powder
(hour) Choline (mg/kg) Choline (mg/kg)
1
1945
559
2
2070
570
3
2104
574
4
2134
577
5
2154
577
22
2173
577
Carn-06 (Choline Validation Report)
FOR ERP USE ONLY
DO NOT DISTRIBUTE