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Appendix 1: Linearity
Choline:
Actual
Concentration
(ng/g)
IS Actual
Concentration
(ng/g)
Area
(counts)
IS Area
(counts)
Concentration
ratio
Area ratio
std 1
4,568
226,0465
3500,1
95414,3 0,020208231 0,036682647
std 2
8,9997
233,9615
7158,5
100386,5 0,038466585 0,071309878
std 3
37,8086
2,17E+02 26736,9
93116,9 0,173867577 0,287132919
std 4
73,3656
230,264 48328,7
92623,6 0,318615155 0,521775092
std 5
144,8371
231,856 96514,2
99629,3 0,624685581 0,968733239
std 6
253,9907
227,1703 164303,6
90238,0 1,118062969 1,820782019
std 7
545,4022
230,9571 313975,4
82948,7 2,361487047 3,785176869
std 8
990,3721
229,4511 538040,9
76108,7 4,316266516 7,069375187
std 9
1535,7123
230,3535 772579,6
71076,6 6,666763474 10,86967718
std 1
4,568
226,0465
3818,0
93352,2 0,020208231 0,040898639
std 2
8,9997
233,9615
6171,6
96396,4 0,038466585 0,064023053
std 3
37,8086
217,4563 26226,1
94439,2 0,173867577 0,277703701
std 4
73,3656
230,264 48128,2
93023,6 0,318615155 0,517376265
std 5
144,8371
231,856 92548,6
92762,0 0,624685581 0,997698961
std 6
253,9907
227,1703 164892,7
91383,4 1,118062969 1,804405624
std 7
545,4022
230,9571 319196,0
85948,8 2,361487047 3,713790633
std 8
990,3721
229,4511 531861,6
76994,4 4,316266516 6,907796781
std 9
1535,7123
230,3535 764571,2
71884,0 6,666763474 10,6361857
SUMMARY OUTPUT
Regression Statistics
Multiple R
0,999881525
R Square
0,999763065
Adjusted R Square 0,999748257
Standard Error
0,057753575
Observations
18
ANAVA
df
SS
MS
F
Significance F
Regression 1 225,1882143 225,1882143 67513,0793 1,95064E-30
Residual
16 0,053367606 0,003335475
Total
17 225,2415819
Coefficients
Std. Error
t Stat
P-value
Lower 95%
Upper 95%
Intercept
-0,004021295 0,017370086 -0,23150690 0,819855739 -0,04084423 0,032801643
Concentration ratio 1,613426982 0,006209482
259,83279 1,95064E-30 1,60026346 1,626590496
RESIDUAL OUTPUT
Observation Predicted Area
Residuals
1
0,028583211 0,008099436
2
0,058041732 0,013268147
3
0,276501345 0,010631574
4
0,510040993 0,011734099
5
1,003863276 -0,035130037
6
1,799891666 0,020890352
Carn-06 (Choline Validation Report)
FOR ERP USE ONLY
DO NOT DISTRIBUTE