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Sample: NIST SRM 1849A
Matrix: Infant/adult nutritional formula.
Species
Result
n Reference value Comment
free choline ion
802 ± 21 mg/kg 4 789 mg/kg
Informative value
Sample: NIST SRM 1546
Matrix: Meat Homogenate.
Species
Result
N Reference value
Comment
choline ion
558 ± 14 mg/kg
2 580
Informative value
For both choline values on SRM 1849A (free) and SRM 1546 (total) the meas-
ured values is in good agreement with the informative values.
Measuring range:
The measuring range in this validation covers the interval: LOQ
–
1000 ng/g.
Samples containing concentration higher than the highest standard will be reana-
lysed with a more fitting sample size and/or dilution leading to a concentration in-
side the standard range.
Limit of detection / quantification:
LOD has been determined by treating a standard at low level (9 ng/g) as a sam-
ple, the reason this approach has been used is that it has been impossible to find
a natural sample containing choline at a level near the expected limit of detection.
The sample was analysed as a 10 replicates measurement on the same day, the
standard deviation (S
r
) has been calculated from these results. See appendix 3.
LOD is calculated as 3 x S
r
and LOQ as (10 x S
r
).
The limit of detection and quantification for choline is calculated to:
LOD: 2,2 ng/g
LOQ: 7,3 ng/g
LOQ in samples as mg/kg is calculated from an aliquot of 20 g sample (maxi-
mum) extracted into 110 g extraction solution. 10 g extraction solution is diluted to
250 g, (this sums up to a total dilution factor of 137,5).
Choline in samples:
LOQ: 1,0 mg/kg
Carn-06 (Choline Validation Report)
FOR ERP USE ONLY
DO NOT DISTRIBUTE