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t / min

0 (free)

30 60 90 120 150 180 240 300 360

From the data (see appendix 4) it is seen that a saponification time of 240 min is

sufficient to convert all bound choline to the free forms for all the tested matrixes.

Choline:

0

500

1000

1500

2000

2500

3000

Choline (mg/kg)

Time (min)

Saponification 100°C

raw egg

Petfood

Infant formula

Cornflakes

Fishfeed

Carn-06 (Choline Validation Report)

FOR ERP USE ONLY

DO NOT DISTRIBUTE