Carn-06_Book

made for every batch and that relative difference (daily average vs. overall aver-

age) is low this is seen as being acceptable.

For choline the RSD% will be set to 5% for choline.

Trueness:

The methods trueness has been evaluated by analysing reference material.

Reference materials:

Reference materials with values on choline exist or are at the moment under test-

ing for certification.

We have analysed the certified reference material SRM 1849A (Infant/adult nutri-

tional formula), SRM 1546 (Meat Homogenate) and SRM 1845A (Whole Egg

powder, under certification).

Sample: NIST SRM 1849A

Matrix: Infant/adult nutritional formula.

Species

Result (m)

n Reference value (CRM) k Comment

choline ion 1041 ± 21 mg/kg 8 1090 ± 110 mg/kg

2 total choline

The certified values have been evaluated according to ERM application Note 1.

Example of the calculation:

The certified content of choline ion is (1090 ± 110) mg/kg,

= 2,00.

The SRM was analysed 8 times (1041 ± 21) mg/kg.

≤

then no significant difference between the measured result and the cer-

tified value has been demonstrated.

Carn-06 (Choline Validation Report)

FOR ERP USE ONLY

DO NOT DISTRIBUTE