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made for every batch and that relative difference (daily average vs. overall aver-
age) is low this is seen as being acceptable.
For choline the RSD% will be set to 5% for choline.
Trueness:
The methods trueness has been evaluated by analysing reference material.
Reference materials:
Reference materials with values on choline exist or are at the moment under test-
ing for certification.
We have analysed the certified reference material SRM 1849A (Infant/adult nutri-
tional formula), SRM 1546 (Meat Homogenate) and SRM 1845A (Whole Egg
powder, under certification).
Sample: NIST SRM 1849A
Matrix: Infant/adult nutritional formula.
Species
Result (m)
n Reference value (CRM) k Comment
choline ion 1041 ± 21 mg/kg 8 1090 ± 110 mg/kg
2 total choline
The certified values have been evaluated according to ERM application Note 1.
1
Example of the calculation:
The certified content of choline ion is (1090 ± 110) mg/kg,
k
= 2,00.
The SRM was analysed 8 times (1041 ± 21) mg/kg.
If
Δ
m
≤
U
Δ
then no significant difference between the measured result and the cer-
tified value has been demonstrated.
1
http://irmm.jrc.ec.europa.eu/html/reference_materials_catalogue/user_support/index.htm.Carn-06 (Choline Validation Report)
FOR ERP USE ONLY
DO NOT DISTRIBUTE