9 / 97
ples of the above mentioned sample types. No interferences have been ob-
served.
Linearity:
The Linearity has been evaluated twice for both compounds with a 9 points cali-
bration.
For the data and the result of the regression analysis performed in excel, see ap-
pendix 1.
Carnitine:
There is seen a very significant correlation between concentration ratio and area
ratio. R
2
=0,9998. The slope of the line is 0,9596 and the intercept with the y-axis
is 0,0046. The calculated 95% confidence interval for the intercept includes 0.
The calibration curve therefore goes through 0.
For residuals plotted against the concentration ratio, it is seen that the points are
randomly distributed around the x-axis (concentration ratio), indicating that the
calibration curve for the concentrations included are linear.
Precision:
For the evaluation of the precision two types of samples have been used:
A milk powder (nutritional formula).
A pet food. (dry pet food)
The repeatability (S
r
) and reproducibility (S
R
) have been evaluated with a variance
analysis model. The data consists of 16 days of double replicate measurements.
The dataset has been evaluated with ANAVA single factor (Excel 2010), see ap-
pendix 2.
Milk powder (mg/kg)
Pet food (mg/kg)
Carnitine
Carnitine
Reproducibility (S
R
)
3,1%
3,3%
Repeatability (S
r
)
2,6%
1,9%
F
1,865
5,188
F
crit
2,352
2,352
For carnitine in the pet food it is seen that there is a significant difference in the
measured results between days. Considering that a new calibration curve is
Carn-06 - Validation Report
FOR ERP USE ONLY
DO NOT DISTRIBUTE