Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

35-POS

Board 18

3D-Structural Modeling by Advanced Electron Microscopy at Whole Single Cell Level

Atsuko H. Iwane

1,2

Osaka University, Suita, Osaka, Japan.

Riken, QBiC, Suita, Osaka, Japan,

Recently several microscopy techniques have revealed the dynamics and structure of

intracellular material such as organelles and supra molecular proteins for a better understanding

of the fundamental properties of a cell. Several advanced electron microscopes provide

information on extremely small biological specimens. Among them, cryo-TEM and tomography

techniques are useful for visualization of fine structures but the observation area is limited.

Conversely, FIB (Focus ion beam)-SEM and SBF (serial block face)-SEM theoretically are not

affected by the thickness of the sample. Furthermore, UHVEM (Ultra-high voltage-TEM)

tomography is expected to be able to visualize the detailed 3D-structure of thylakoid membrane

in the plastid and of the micro-compartments (cristae) in mitochondria. In this meeting, while

taking advantage of these characteristics using these advanced electron microscopy techniques I

will introduce to 3D-structural modeling of the interaction of intracellular organelles and the

internal fine structure of these organelles of

C. merolae

cells (2-5 microns), which is considered

as the primitive eukaryotic organism during cell division at the whole cell level. In particular, I

definitely would like to discuss about transitional changes in thylakoid membranes in plastid

with the researcher of computer simulation modeling.