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3M MDA2 E. coli O157:H7 Collaborative Study
May 2016
OMA-2016-May-XXXX
DRAFT DOCUMENT
12
2.3.
For all test portions, follow the isolation procedure using Dynal #710.04
E.coli
O157:H7
1
immunomagnetic beads as described in USDA/FSIS.
2
2.4.
Prepare the beads as described by USDA/FSIS, spread plate dilutions of the bead suspension
3
(with and without acid treatment) onto modfied Rainbow® Agar. Incubate for 20-24 h at
4
35
2
C.
5
2.5.
Confirm a minimum of 1 typical colony from all potential positive test portions with typical
6
colonies following the identification and confirmation procedures as per USDA FSIS.
7
8
Final Results
9
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All results are to be recorded on the data sheets provided. For each test portion, record the results
11
reported by the 3M method along with the confirmation results for both the 3M MDA2 -
E. coli
O157
12
(including H7) and reference methods. Immediately upon completion of each food type, the data sheet
13
should be faxed or emailed to TBD, co-Study Director.
14
OMAMAN-35 B: Collaborative Study Protocol
ERP USE ONLY
January 2017
AOAC Research Institute
Expert Review Panel Use Only